Typical Testing Data/Standard Curve (for reference only)
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Acetyl-CoA Carboxylase Assay Kit
Acetyl-CoA Carboxylase Microplate Assay Kit
Synonyms
Acetyl-CoA Carboxylase; Acetyl-CoA Carboxylase Microplate Assay Kit; Acetyl-CoA Carboxylase assay kit
Samples
Tissue extracts, cell lysate, cell culture media and other biological fluids samples
Detection Range
1 umol/L - 100 umol/L
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for Acetyl-CoA Carboxylase assay kit
Background/Introduction: Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes the irreversible carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes and in the chloroplasts of most plants and algae, whereas it is a large, multi-domain enzyme in the endoplasmic reticulum of most eukaryotes. The most important function of ACC is to provide the malonyl-CoA substrate for the biosynthesis of fatty acids. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. Acetyl-CoA carboxylase can react with acetyl-CoA and ATP, the products are malonyl-CoA, ADP and inorganic phosphorus. The Acetyl-CoA carboxylase activity will be determined by the increase of inorganic phosphorus. At the end of the reaction period, the dye reagent forms a color with released phosphate, which is measured on a plate reader 635 nm.
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Product Notes
The Acetyl-CoA Carboxylase (Catalog #AAA8303295) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Acetyl-CoA Carboxylase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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