Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Acetyl-CoA carboxylase 1 (ACACA) Standard Curve.)
Rat Acetyl-CoA Carboxylase 1 (ACACA) ELISA Kit | ACACA elisa kit
Rat Acetyl-CoA Carboxylase 1 (ACACA) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Acetyl-CoA carboxylase 1 (ACACA) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Acetyl-CoA carboxylase 1 (ACACA) Standard Curve.)
Background: Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes the irreversible carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes and in the chloroplasts of most plants and algae, whereas it is a large, multi-domain enzyme in the endoplasmic reticulum of most eukaryotes. The most important function of ACC is to provide the malonyl-CoA substrate for the biosynthesis of fatty acids. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. The human genome contains the genes for two different ACCs — ACACA and ACACB. In muscle cells, malonyl-CoA inhibits beta-oxidation.
NCBI and Uniprot Product Information
NCBI Description
catalyzes the rate-limiting carboxylation of acetyl CoA to malonyl CoA during long-chain fatty acid synthesis [RGD, Feb 2006]
Uniprot Description
ACC1: a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Phosphorylation by AMPK or PKA inhibits the enzymatic activity of ACC. ACC-alpha is the predominant isoform in liver, adipocyte and mammary gland. ACC-beta is the major isoform in skeletal muscle and heart. Phosphorylation regulates its activity.
Protein type: Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; EC 6.3.4.14; EC 6.4.1.2; Ligase; Lipid Metabolism - fatty acid biosynthesis
Chromosomal Location of Human Ortholog: 10q26
Cellular Component: actin cytoskeleton; cytosol; extracellular exosome; fibrillar center; mitochondrion
Molecular Function: acetyl-CoA carboxylase activity; ATP binding; biotin binding; biotin carboxylase activity; identical protein binding; kinase binding; metal ion binding
Biological Process: acetyl-CoA metabolic process; cellular response to prostaglandin E stimulus; fatty acid biosynthetic process; lipid homeostasis; lipid metabolic process; malonyl-CoA biosynthetic process; multicellular organismal protein metabolic process; protein homotetramerization; response to drug; response to organic cyclic compound; tissue homeostasis
Research Articles on ACACA
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Product Notes
The Rat ACACA acaca (Catalog #AAA9711945) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711945 ELISA Kit recognizes Rat ACACA. It is sometimes possible for the material contained within the vial of "Acetyl-CoA Carboxylase 1 (ACACA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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