Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Acetyl-CoA carboxylase 1 (ACACA) Standard Curve.)
Mouse Acetyl-CoA Carboxylase 1 (ACACA) ELISA Kit | ACACA elisa kit
Mouse Acetyl-CoA Carboxylase 1 (ACACA) ELISA Kit
Gene Names
Acaca; Acac; Acc1; Gm738; A530025K05Rik
Reactivity
Mouse
Synonyms
Acetyl-CoA Carboxylase 1 (ACACA); Mouse Acetyl-CoA Carboxylase 1 (ACACA) ELISA Kit; ACACA; ACAC; ACC; ACC1; ACCA; ACC-alpha; acetyl-CoA carboxylase 1; acetyl-CoA carboxylase-alpha; ACACA elisa kit
Reactivity
Mouse
Specificity
Mouse Acetyl-CoA carboxylase 1 (ACACA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ACACA. No significant cross-reactivity or interference between Mouse ACACA and analogues was observed.
Sequence Length
2345
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Acetyl-CoA carboxylase 1 (ACACA) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Acetyl-CoA carboxylase 1 (ACACA) Standard Curve.)
Related Product Information for ACACA elisa kit
Description: This Mouse Acetyl-CoA carboxylase 1 (ACACA) ELISA Kit employs a two-site sandwich ELISA to quantitate ACACA in samples. An antibody specific for ACACA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACACA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACACA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACACA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes the irreversible carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes and in the chloroplasts of most plants and algae, whereas it is a large, multi-domain enzyme in the endoplasmic reticulum of most eukaryotes. The most important function of ACC is to provide the malonyl-CoA substrate for the biosynthesis of fatty acids. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. The human genome contains the genes for two different ACCs — ACACA and ACACB. In muscle cells, malonyl-CoA inhibits beta-oxidation.
Background: Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes the irreversible carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes and in the chloroplasts of most plants and algae, whereas it is a large, multi-domain enzyme in the endoplasmic reticulum of most eukaryotes. The most important function of ACC is to provide the malonyl-CoA substrate for the biosynthesis of fatty acids. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. The human genome contains the genes for two different ACCs — ACACA and ACACB. In muscle cells, malonyl-CoA inhibits beta-oxidation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
acetyl-CoA carboxylase 1
NCBI Official Synonym Full Names
acetyl-Coenzyme A carboxylase alpha
NCBI Official Symbol
Acaca
NCBI Official Synonym Symbols
Acac; Acc1; Gm738; A530025K05Rik
NCBI Protein Information
acetyl-CoA carboxylase 1
UniProt Protein Name
Acetyl-CoA carboxylase 1
UniProt Gene Name
Acaca
UniProt Synonym Gene Names
ACC1
Uniprot Description
Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
Research Articles on ACACA
Similar Products
Product Notes
The Mouse ACACA acaca (Catalog #AAA9717142) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717142 ELISA Kit recognizes Mouse ACACA. It is sometimes possible for the material contained within the vial of "Acetyl-CoA Carboxylase 1 (ACACA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
