FBP1 recombinant protein

FBP1, 1-338aa, Human, His tag, E Coli

Cat. Num. AAA203602

• Gene Names: FBP1; FBP
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: FBP1; 1-338aa; Human; His tag; E Coli; FBPase1; FBP; D fructose 1 6 bisphosphate 1 phosphohydrolase 1; EC 3.1.3.11; FBP 1; FBPase 1; Fructose 1 6 bisphosphatase 1 Fructose bisphosphatase 1; Growth inhibiting protein 17; Liver fructose bisphosphatase.; FBP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MADQAPFDTD VNTLTRFVME EGRKARGTGE LTQLLNSLCT AVKAISSAVR KAGIAHLYGI AGSTNVTGDQ VKKLDVLSND LVMNMLKSSF ATCVLVSEED KHAIIVEPEK RGKYVVCFDP LDGSSNIDCL VSVGTIFGIY RKKSTDEPSE KDALQPGRNL VAAGYALYGS ATMLVLAMDC GVNCFMLDPA IGEFILVDKD VKIKKKGKIY SLNEGYARDF DPAVTEYIQR KKFPPDNSAP YGARYVGSMV ADVHRTLVYG GIFLYPANKK SPNGKLRLLY ECNPMAYVME KAGGMATTGK EAVLDVIPTD IHQRAPVILG SPDDVLEFLK VYEKHSAQ
• Sequence Length: 338

• Applicable Applications for FBP1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Domain: 1-338aa
• Preparation and Storage: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for FBP1 recombinant protein

Fructose-1,6-bisphosphatase 1 (FBP1) is a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1,6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. Recombinant FBP1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for FBP1 recombinant protein

Metabolism

References

Rabenhorst u., et al. (2009) Hepatology. 50 (4):1121-9. ; Hirota K., et al. (2008) Nature. 456 (7218):130-4.;

NCBI and Uniprot Product Information

• NCBI GI #: 16579888
• NCBI GeneID: 2203
• NCBI Accession #: NP_000498.2
• NCBI GenBank Nucleotide #: NM_000507.3
• UniProt Accession #: P09467; O75571; Q53F94; Q96E46
• Molecular Weight: 39.0 kDa (358aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: fructose-1,6-bisphosphatase 1
• NCBI Official Synonym Full Names: fructose-bisphosphatase 1
• NCBI Official Symbol: FBP1
• NCBI Official Synonym Symbols: FBP
• NCBI Protein Information: fructose-1,6-bisphosphatase 1
• UniProt Protein Name: Fructose-1,6-bisphosphatase 1
• Protein Family: Fat-body protein
• UniProt Gene Name: FBP1
• UniProt Synonym Gene Names: FBP; FBPase 1

NCBI Description

Fructose-1,6-bisphosphatase 1, a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1,6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain.