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Western Blot (WB) ((0.05ug/ml) staining of Mouse Liver (A) and Rat Liver (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat Arginase, type 1/arg1 Polyclonal Antibody | anti-Arg1 antibody

Goat anti-Arginase, type 1/arg1(rat) Antibody

Reactivity
Tested: Mouse, Rat; Expected from sequence similarity: Mouse, Rat
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Arginase; type 1/arg1; Polyclonal Antibody; Goat anti-Arginase; type 1/arg1(rat) Antibody; Arg1; arginase 1; AI type I arginase; arginase 1 liver; Arg1 antibody; arginase 1 antibody; AI type I arginase antibody; arginase 1 liver antibody; type 1; type 1 / arg1(rat); anti-Arg1 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Mouse, Rat; Expected from sequence similarity: Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
NHKPETDYLKPPK
Sequence Length
323
Applicable Applications for anti-Arg1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 1000.
Western Blot: Approx 37kDa band observed in Mouse and Rat Liver lysates (calculated MW of 34.8kDa according to mouse NP_031508.1). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-NHKPETDYLKPPK, from the C Terminus of the protein sequence according to NP_058830.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.05ug/ml) staining of Mouse Liver (A) and Rat Liver (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.05ug/ml) staining of Mouse Liver (A) and Rat Liver (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,973 Da
NCBI Official Full Name
arginase-1
NCBI Official Synonym Full Names
arginase 1
NCBI Official Symbol
Arg1
NCBI Protein Information
arginase-1
UniProt Protein Name
Arginase-1
Protein Family
UniProt Gene Name
Arg1
UniProt Entry Name
ARGI1_RAT

NCBI Description

catalyzes the hydrolysis of arginine to ornithine and urea in arginine metabolism; regulates nitric oxide production [RGD, Feb 2006]

Uniprot Description

ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.3.1; Amino Acid Metabolism - arginine and proline; Hydrolase

Cellular Component: cell soma; cytoplasm; extracellular space; mitochondrial outer membrane; neuron projection; nucleus

Molecular Function: arginase activity; manganese ion binding

Biological Process: aging; arginine catabolic process to ornithine; arginine metabolic process; collagen biosynthetic process; female pregnancy; liver development; lung development; mammary gland involution; maternal process involved in pregnancy; positive regulation of endothelial cell proliferation; response to amine stimulus; response to amino acid stimulus; response to axon injury; response to cadmium ion; response to drug; response to herbicide; response to lipopolysaccharide; response to manganese ion; response to methylmercury; response to peptide hormone stimulus; response to selenium ion; response to steroid hormone stimulus; response to vitamin A; response to vitamin E; response to wounding; response to zinc ion; urea cycle

Research Articles on Arg1

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Product Notes

The Arg1 arg1 (Catalog #AAA421477) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Arginase, type 1/arg1(rat) Antibody reacts with Tested: Mouse, Rat; Expected from sequence similarity: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Arginase, type 1/arg1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 1000. Western Blot: Approx 37kDa band observed in Mouse and Rat Liver lysates (calculated MW of 34.8kDa according to mouse NP_031508.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the Arg1 arg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NHKPETDYLK PPK. It is sometimes possible for the material contained within the vial of "Arginase, type 1/arg1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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