Immunohistochemistry (IHC)
(Immunochemical staining of human PLA2G4F in human skin with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human PLA2G4F Polyclonal Antibody | anti-PLA2G4F antibody
Anti-PLA2G4F Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
PLA2G4F; Polyclonal Antibody; Anti-PLA2G4F Antibody; Rabbit Polyclonal; PLA2G4F Antibody; Rabbit PAb; Antigen Affinity Purified; phospholipase A2; group IVF; Anti-PLA2G4FZ Antibody; anti-PLA2G4F antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PLA2G4F
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PLA2G4F antibody
Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
IHC-P: 1:50-1:200
ICC/IF: 1:100-1:500
ICC/IF: 1:100-1:500
Immunogen
E Coli-derived Human PLA2G4F fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PLA2G4F fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human PLA2G4F in human skin with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PLA2G4F in human skin with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunofluorescence (IF)
(Immunofluorescence staining of PLA2G4F in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PLA2G4F polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of PLA2G4F in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PLA2G4F polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.)
Related Product Information for anti-PLA2G4F antibody
PLA2G4F (Phospholipase A2 Group IVF) is a Protein Coding gene. The PLA2G4F gene, located on 15q15.1, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. Pla2g4f is a calcium-dependent phospholipase A2 that has three potentially significant nsSNP variants and showed expression differences across mouse strains. PLA2G4F has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine. PLA2G4F produces arachidonic acid, which is a substrate for lipoxygenases and, in turn, for GPXs. Diseases associated with PLA2G4F include Ileocolitis and Inflammatory Bowel Disease 9. Among its related pathways are Sweet Taste Signaling and Acyl chain remodeling of PE. 180 organisms have orthologs with the human gene PLA2G4F.
References
Ohto T, et al. (2005) Identification of novel cytosolic phospholipase a(2)s, murine cpla(2){delta}, {epsilon}, and {zeta}, which form a gene cluster with cpla(2){beta}. J Biol Chem 280 (26): 24576-24583.Mavoungou E, et al. (1997) Hiv and siv envelope glycoproteins induce phospholipase a2 activation in human and macaque lymphocytes. J Acquir Immune Defic Syndr Hum Retrovirol 16 (1): 1-9.Esworthy RS, et al. (2012) Analysis of candidate colitis genes in the gdac1 locus of mice deficient in glutathione peroxidase-1 and -2. PLoS One 7 (9): e44262.
Similar Products
Product Notes
The PLA2G4F (Catalog #AAA8108099) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PLA2G4F Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLA2G4F can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). IHC-P: 1:50-1:200 ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the PLA2G4F for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLA2G4F, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
