General Acid Phosphatase Assay Kit | PHOA assay kit
Acid Phosphatase Assay Kit
Reactivity
General
Applications
Functional Assay
Synonyms
Acid Phosphatase; Acid Phosphatase Assay Kit; PHOA assay kit
Reactivity
General
Sequence Length
412
Applicable Applications for PHOA assay kit
Functional Assay
Samples
Tissue Extracts, Cell Lysate, Cell Culture Media And Other Biological Fluids Samples
Detection Range
1 umol/L-100 umol/L
Preparation and Storage
Shipped and store at 4 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PHOA assay kit
Background/Introduction: Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes the irreversible carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes and in the chloroplasts of most plants and algae, whereas it is a large, multi-domain enzyme in the endoplasmic reticulum of most eukaryotes. The most important function of ACC is to provide the malonyl-CoA substrate for the biosynthesis of fatty acids. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. Acetyl-CoA carboxylase can react with acetyl-CoA and ATP, the products are malonyl-CoA, ADP and inorganic phosphorus. The Acetyl-CoA carboxylase activity will be determined by the increase of inorganic phosphorus. At the end of the reaction period, the dye reagent forms a color with released phosphate, which is measured on a plate reader 635 nm.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
45,628 Da
NCBI Official Full Name
acid phosphatase
UniProt Protein Name
Phosphate-repressible acid phosphatase
Protein Family
UniProt Gene Name
PHOA
UniProt Entry Name
PHOA_PENCH
