Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western Blot analysis of Hela cells with Jagged1 Polyclonal Antibody.)

Notch Receptor Interaction Antibody Kit

Notch Receptor Interaction Antibody Sampler Kit

Applications
Western Blot
Synonyms
Notch Receptor Interaction; Notch Receptor Interaction Antibody Sampler Kit; Notch Receptor Interaction antibody kit
Ordering
For Research Use Only!
Applicable Applications for Notch Receptor Interaction antibody kit
Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of Hela cells with Jagged1 Polyclonal Antibody.)

Western Blot (WB) (Western Blot analysis of Hela cells with Jagged1 Polyclonal Antibody.)

Western Blot (WB)

(Western Blot analysis of A549 cells with NUMB Polyclonal Antibody.)

Western Blot (WB) (Western Blot analysis of A549 cells with NUMB Polyclonal Antibody.)
Related Product Information for Notch Receptor Interaction antibody kit
Background: Notch signaling is activated upon engagement of the Notch receptor with its ligands, the Delta, Serrate, Lag2 (DSL) single-pass type I membrane proteins. DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch. Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3, and 4. Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the gamma- secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a similiar manner, suggesting bi-directional signaling through receptor-ligand interactions. TNF-alpha converting enzyme (TACE), also known as ADAM17, is a transmembrane metalloprotease that plays a key role in the cleavage of a number cell surface molecules in a process known as "shedding". TACE is abundantly expressed in many adult tissues, but in fetal development, expression is differentially regulated. TACE activates Notch in a ligand-independent manner and has been shown to play a role in the development of the Drosophila nervous system. Recombining Binding Protein, SUppressor of Hairless (RBPSUH), also termed RBP-J or CSL, is the DNA-binding component of the transcription complex regulated by canonical Notch signaling. In the absence of Notch activation, RBPSUH suppresses target gene expression through interactions with a co-repressor complex containing histone deacetylase. Upon activation of Notch receptors, the Notch intracellular domain (NICD) translocates to the nucleus and binds to RBPSUH. This displaces the co-repressor complex and replaces it with a transcription activation complex that includes Mastermind-like (MAML) proteins and histone acetylase p300, leading to transcriptional activation of Notch target genes. Numb contains an amino-terminal phosphotyrosine- binding (PTB) domain and carboxy-terminal endocytic binding motifs for alpha-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis. There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions. Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch. The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates.

Similar Products

Product Notes

The Notch Receptor Interaction (Catalog #AAA2549974) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Notch Receptor Interaction can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Notch Receptor Interaction for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Notch Receptor Interaction, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.