Rabbit Notch 1 Polyclonal Antibody | anti-Notch1 antibody

Notch 1 Antibody

Cat. Num. AAA841160

• Gene Names: Notch1; N1; Mis6; Tan1; lin-12; 9930111A19Rik
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: Notch 1; Polyclonal Antibody; Notch 1 Antibody; Notch-1; NOTCH1; TAN1; hN1; anti-Notch1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 0.5mg/ml affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
• Sequence Length: 2531

• Applicable Applications for anti-Notch1 antibody: Western Blot (WB)
• Application Notes: Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes a 120 kDa band from samples of human and mouse origins. Reactivity to other species has not been tested.
• Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 1760 of mouse Notch1
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: Store at -20°C. For long term storage, aiquot and freeze at -70°. Avoid repeated freeze/defrost cycles.

Testing Data

(Western Blot analysis of Notch 1 in Jurkat (Lane 1) and 3T3 (Lane 2) cell lysate.)

Related Product Information for anti-Notch1 antibody

Background: Notch proteins are transmembrane receptor that regluate cell fate decisions. Four Notch homologs have been identified in mammals namely Notch 1, Notch 2, Notch 3 and Notch 4. The membrane ligand for Notch includes Jagged 1, Jagged 2 and Delta. Notch 1 is a 270 kDa transmembrane receptor that can be activated by Delta (Dl) ligand. Activation of Notch 1 will result in the proteolytic cleavage of Notch 1 into a 120 kDa length protein.

NCBI and Uniprot Product Information

• NCBI GI #: 224967065
• NCBI GeneID: 18128
• NCBI Accession #: NP_032740
• NCBI GenBank Nucleotide #: NM_008714.3
• NCBI Official Full Name: neurogenic locus notch homolog protein 1
• NCBI Official Synonym Full Names: notch 1
• NCBI Official Symbol: Notch1
• NCBI Official Synonym Symbols: N1; Mis6; Tan1; lin-12; 9930111A19Rik
• NCBI Protein Information: neurogenic locus notch homolog protein 1
• UniProt Protein Name: Neurogenic locus notch homolog protein 1
• Protein Family: Neurogenic locus notch homolog protein
• UniProt Gene Name: Notch1
• UniProt Synonym Gene Names: Motch; Notch 1; NEXT; NICD
• UniProt Entry Name: NOTC1_MOUSE

Uniprot Description

Notch 1: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Forms a trimeric complex with FBXW7 and SGK1. Interacts with HIF1AN. HIF1AN negatively regulates the function of notch intracellular domain (NICD), accelerating myogenic differentiation. In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues. Belongs to the NOTCH family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, misc.; Oncoprotein; Transcription factor

Cellular Component: Golgi apparatus; cell surface; membrane; cell; integral to plasma membrane; cytoplasm; acrosome; plasma membrane; integral to membrane; nucleus; receptor complex

Molecular Function: enzyme inhibitor activity; protein binding; enzyme binding; chromatin DNA binding; sequence-specific DNA binding; metal ion binding; receptor activity; chromatin binding; calcium ion binding; transcription factor activity

Biological Process: neural tube development; negative regulation of calcium ion-dependent exocytosis; positive regulation of apoptosis; heart development; multicellular organismal development; positive regulation of transcription, DNA-dependent; positive regulation of JAK-STAT cascade; positive regulation of glial cell differentiation; regulation of cell migration; negative regulation of BMP signaling pathway; compartment specification; neuron differentiation; positive regulation of endothelial cell differentiation; negative regulation of ossification; somatic stem cell division; negative regulation of osteoblast differentiation; positive regulation of cardiac muscle cell proliferation; embryonic limb morphogenesis; positive regulation of keratinocyte differentiation; positive regulation of astrocyte differentiation; negative regulation of photoreceptor cell differentiation; positive regulation of neuroblast proliferation; cell fate commitment; transcription, DNA-dependent; neuron fate commitment; keratinocyte differentiation; regulation of transcription from RNA polymerase II promoter; negative regulation of neuron differentiation; branching morphogenesis of a tube; regulation of gene expression; response to muramyl dipeptide; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; determination of left/right symmetry; anagen; positive regulation of epithelial cell proliferation; foregut morphogenesis; endoderm development; osteoblast fate commitment; transcription from RNA polymerase II promoter; epidermis development; cell fate specification; cardiac muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; regulation of epithelial cell proliferation; negative regulation of neurogenesis; negative regulation of cell proliferation; glial cell differentiation; regulation of neurogenesis; regulation of transcription, DNA-dependent; astrocyte differentiation; cardiac muscle morphogensis; forebrain development; positive regulation of cell proliferation; angiogenesis; heart looping; regulation of somitogenesis; cell differentiation; positive regulation of BMP signaling pathway; regulation of developmental process; mesenchymal cell development; Notch signaling pathway; hair follicle morphogenesis; in utero embryonic development; lumen formation; liver development; humoral immune response; activation of Notch receptor target transcription factor; regulation of cell proliferation; negative regulation of oligodendrocyte differentiation; negative regulation of cell differentiation; inflammatory response to antigenic stimulus; axonogenesis; negative regulation of catalytic activity; epithelial to mesenchymal transition; spermatogenesis; sprouting angiogenesis; negative regulation of myoblast differentiation; auditory receptor cell fate commitment; lung development; positive regulation of cell migration