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Western Blot (WB)
(Western Blot analysis of Mouse brain, Rat musle using Cleaved-Notch 1 (V1754) Polyclonal Antibody at dilution of 1:500.)
Notch Activated Targets Antibody Kit
Notch Activated Targets Antibody Sampler Kit
Applications
Western Blot
Synonyms
Notch Activated Targets; Notch Activated Targets Antibody Sampler Kit; Notch Activated Targets antibody kit
Applicable Applications for Notch Activated Targets antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of Mouse brain, Rat musle using Cleaved-Notch 1 (V1754) Polyclonal Antibody at dilution of 1:500.)
Western Blot (WB)
(Western Blot analysis of Mouse brain, Rat musle using Cleaved-Notch 1 (V1754) Polyclonal Antibody at dilution of 1:500.)
Western Blot (WB)
(Western Blot analysis of HT-29 cell using NOTCH1 Polyclonal Antibody at dilution of 1:600)
Western Blot (WB)
(Western Blot analysis of HT-29 cell using NOTCH1 Polyclonal Antibody at dilution of 1:600)
Western Blot (WB)
(Western Blot analysis of K562 cells with c-Myc Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of K562 cells with c-Myc Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of COLO205 cells with p21 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of COLO205 cells with p21 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of COS-7 cells with Cyclin D3 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of COS-7 cells with Cyclin D3 Polyclonal Antibody.)
Related Product Information for Notch Activated Targets antibody kit
Background: Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate. Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand- binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes. RBPSUH (Recombining Binding Protein, SUppressor of Hairless), is the DNA- binding component of the transcription complex regulated by canonical Notch signaling. Binding of Notch with RBPSUH activates a transcription activation complex that includes Mastermind-like (MAML) proteins, leading to transcriptional activation of Notch target genes. The NICD binds and activates c-Myc which functions as a transcriptional regulator with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis. The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. The NICD- RBPSUH complex binds and activates p21 for transcription. HES1 (Hairy and Enhancer of Split 1) is one of seven members of the HES family of basic helix-loop-helix (bHLH) transcription factors that is particularly well known as a repressive mediator of the canonical Notch signaling pathway. HES1 plays a key role in mediating Notch-dependent T cell lineage commitment, and has been reported to be an essential mediator of Notch-induced T cell acute lymphoblastic leukemia (T-ALL). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression. Transcription of cyclin D is in part regulated by the NICD binding to the promoter region of cyclin D.
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Product Notes
The Notch Activated Targets (Catalog #AAA2550024) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Notch Activated Targets can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Notch Activated Targets for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Notch Activated Targets, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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