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Autophagy Atg8 Family Antibody Kit
Autophagy Atg8 Family Antibody Sampler Kit
Applications
Western Blot
Synonyms
Autophagy Atg8 Family; Autophagy Atg8 Family Antibody Sampler Kit; Autophagy Atg8 Family antibody kit
Applicable Applications for Autophagy Atg8 Family antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) 3T3, 3) Rat brain using LC3A Monoclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) 3T3, 3) Rat brain with MAP LC3B Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of 823 and Raji cell using MAP1LC3C Polyclonal Antibody at dilution of 1:450)
Western Blot (WB)
(Western Blot analysis of Mouse brain tissue using GABARAPL1 Monoclonal Antibody at dilution of 1:2000.)
Related Product Information for Autophagy Atg8 Family antibody kit
Background: Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes, including development, differentiation, neurodegenerative diseases, infection, and cancer. Atg8 is a ubiquitin-like protein that is critical for autophagosome formation. Atg8 is synthesized as a precursor protein that is processed by the cysteine protease Atg4, followed by lipidation with phosphatidylethanolamine (PE) in a ubiqutin-like conjugation pathway involving Atg7 and Atg3. This processing of Atg8, which is described as a conversion from type-I to type-II forms, is frequently described as a marker for autophagy. The type-II form of Atg8 is incorporated into maturing autophagosomes and leads to the recruitment of additional autophagy components, including cargo receptors like SQSTM1/p62. While yeast has a single Atg8 gene, many eukaryotes have at least six orthologs, including three microtubule-associated protein 1 light chain 3 (MAP1LC3/LC3) family members (LC3A, LC3B, and LC3C) and three GABAA receptor associated protein (GABARAP) family members (GABARAP, GABARAPL1/GEC1, and GABARAPL2/GATE-16). While highly conserved, these various family members can have important differences in their post-translational processing, expression profile, and protein interactions including distinct cargo receptor. This complexity within the Atg8 family is critical for selective mechanisms of autophagy that have been reported.
