Rabbit anti-Human, Rat ATG4B Polyclonal Antibody | anti-ATG4B antibody

ATG4B Polyclonal Antibody

Cat. Num. AAA3007453

• Gene Names: ATG4B; APG4B; AUTL1
• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: ATG4B; Polyclonal Antibody; ATG4B Polyclonal Antibody; Cysteine protease ATG4B; AUT-like 1 cysteine endopeptidase; Autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; hAPG4B; anti-ATG4B antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ATG4B polyclonal antibody detects endogenous levels of ATG4B protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 393

• Applicable Applications for anti-ATG4B antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 75-119 of Human ATG4B
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ATG4B pAb at 1:500 dilutionLane1:C6 whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:DLD whole cell lysate (40ug) Lane4:U-87MG whole cell lysate (40ug))

Related Product Information for anti-ATG4B antibody

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Control of autophagy was largely discovered in yeast and involves proteins encoded by a set of autophagy-related genes (Atg). Formation of autophagic vesicles requires a pair of essential ubiquitin-like conjugation systems, Atg12-Atg5 and Atg8-phosphatidylethanolamine (Atg8-PE), which are widely conserved in eukaryotes. Numerous mammalian counterparts to yeast Atg proteins have been described, including three Atg8 proteins (GATE-16, GABARAP, and LC3) and four Atg4 homologs (Atg4A/autophagin-2, Atg4B/autophagin-1, Atg4C/autophagin-3, and Atg4D/autophagin-4). The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homolog for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homolog is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homolog.While Atg4B displays a broad specificity for Atg8 homologues, it preferentially cleaves LC3. Mutation in the corresponding Atg4B gene can be associated with strong inhibition of autophagosome formation. An excess of inactive Atg4B blocks lipidation of Atg8 homologues and inhibits autophagy. This makes Atg4B a potential tool for characterization of the isolation membrane and other autophagy studies.

NCBI and Uniprot Product Information

• NCBI GI #: 47132611
• NCBI GeneID: 23192
• NCBI Accession #: NP_037457.3
• NCBI GenBank Nucleotide #: NP_037457.3
• UniProt Accession #: Q9Y4P1
• Molecular Weight: ~ 48kDa
• NCBI Official Full Name: cysteine protease ATG4B isoform a
• NCBI Official Synonym Full Names: autophagy related 4B cysteine peptidase
• NCBI Official Symbol: ATG4B
• NCBI Official Synonym Symbols: APG4B; AUTL1
• NCBI Protein Information: cysteine protease ATG4B
• UniProt Protein Name: Cysteine protease ATG4B
• Protein Family: Cysteine protease
• UniProt Gene Name: ATG4B
• UniProt Synonym Gene Names: APG4B; AUTL1; KIAA0943; hAPG4B
• UniProt Entry Name: ATG4B_HUMAN

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

ATG4B: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Protease; EC 3.4.22.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; endopeptidase activity; cysteine-type peptidase activity

Biological Process: protein delipidation; mitochondrion degradation; positive regulation of protein catabolic process; autophagy; C-terminal protein lipidation; protein processing; protein targeting to membrane; proteolysis; positive regulation of autophagy; autophagic vacuole formation; cellular response to nitrogen starvation