Western Blot (WB)
(Western Blot analysis of Jurkat cells with Atg4b Polyclonal Antibody.)
Autophagy Vesicle Elongation (LC3 Conjugation) Antibody Kit
Autophagy Vesicle Elongation (LC3 Conjugation) Antibody Sampler Kit
Applications
Western Blot
Synonyms
Autophagy Vesicle Elongation (LC3 Conjugation); Autophagy Vesicle Elongation (LC3 Conjugation) Antibody Sampler Kit; Autophagy Vesicle Elongation (LC3 Conjugation) antibody kit
Applicable Applications for Autophagy Vesicle Elongation (LC3 Conjugation) antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of Jurkat cells with Atg4b Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of Jurkat cells with Atg4b Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of COLO205 cells using Atg4A Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of COLO205 cells using Atg4A Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of K562 cell, Mouse kidney tissue and Raji cell using ATG3 Polyclonal Antibody at dilution of 1:325)
Western Blot (WB)
(Western Blot analysis of K562 cell, Mouse kidney tissue and Raji cell using ATG3 Polyclonal Antibody at dilution of 1:325)
Related Product Information for Autophagy Vesicle Elongation (LC3 Conjugation) antibody kit
Background: Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer. Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin- like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles. The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy. Numerous mammalian counterparts to yeast Atg proteins have been described, including three Atg8 proteins (GATE-16, GABARAP, and LC3) and four Atg4 homologs (Atg4A/autophagin-2, Atg4B/autophagin-1, Atg4C/autophagin-3, and Atg4D/autophagin-4). The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. GABAA receptor associated protein (GABARAP) is an Atg8 family protein with a key role in autophagy, which was originally discovered as a protein associated with the GABAA receptor regulating receptor trafficking to the plasma membrane. Processing of GABARAP involves cleavage by Atg4 family members followed by conjugation by the E1 and E2 like enzymes Atg7 and Atg3.
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Product Notes
The Autophagy Vesicle Elongation (LC3 Conjugation) (Catalog #AAA2549993) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Autophagy Vesicle Elongation (LC3 Conjugation) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Autophagy Vesicle Elongation (LC3 Conjugation) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Autophagy Vesicle Elongation (LC3 Conjugation), Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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