<
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) Jurkat, 3) HepG2, 4) Mouse liver, 5) Rat brain using Glucocorticoid receptor Polyclonal Antibody at dilution of 1:2000.)
Nuclear Receptor Antibody Kit | vdr-a antibody kit
Nuclear Receptor Antibody Sampler Kit
Applications
Western Blot
Synonyms
Nuclear Receptor; Nuclear Receptor Antibody Sampler Kit; vdr-a antibody kit
Sequence Length
532
Applicable Applications for vdr-a antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) Jurkat, 3) HepG2, 4) Mouse liver, 5) Rat brain using Glucocorticoid receptor Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) Jurkat, 3) HepG2, 4) Mouse liver, 5) Rat brain using Glucocorticoid receptor Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of Hela cells with PR Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of Hela cells with PR Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of 823 cells using AR Polyclonal Antibody at dilution of 1:500.)
Western Blot (WB)
(Western Blot analysis of 823 cells using AR Polyclonal Antibody at dilution of 1:500.)
Western Blot (WB)
(Western Blot analysis of HepG2 cells using ERalpha Polyclonal Antibody at dilution of 1:500.)
Western Blot (WB)
(Western Blot analysis of HepG2 cells using ERalpha Polyclonal Antibody at dilution of 1:500.)
Western Blot (WB)
(Western Blot analysis of HuvEc cells using PPAR-gamma Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of HuvEc cells using PPAR-gamma Polyclonal Antibody at dilution of 1:1000.)
Related Product Information for vdr-a antibody kit
Background: Nuclear Receptors are transcription factors responsible for sensing bioactive molecules, including steroid and thyroid hormones. They are regulated by multiple posttranslational modifications, which in turn impacts their ability to regulate the expression of specific genes involved in the control of reproduction, development, and metabolism. Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding. This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. AR plays a crucial role in several stages of male development and the progression of prostate cancer. Estrogen receptor alpha (ERalpha), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains. Through its estrogen-independent and estrogen- dependent activation domains (AF-1 and AF-2, respectively), ERalpha regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Glucocorticoid hormones control cellular proliferation, inflammation, and metabolism through their association with the glucocorticoid receptor (GR)/NR3C1, a member of the nuclear hormone receptor superfamily of transcription factors. Peroxisome proliferator-activated receptor gamma (PPARgamma) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator. PPARgamma is preferentially expressed in adipocytes, as well as in vascular smooth muscle cells and macrophages. Besides its role in mediating adipogenesis and lipid metabolism, PPARgamma also modulates insulin sensitivity, cell proliferation, and inflammation. Human progesterone receptor (PR) is expressed as two forms: the full length PR B and the short form PR A. PR A lacks the first 164 amino acid residues of PR B. Both PR A and PR B are ligand activated, but differ in their relative ability to activate target gene transcription (12,13). Nuclear retinoic acid receptors (RARs) consist of three subtypes encoded by separate genes: alpha (NR1B1), beta (NR1B2), and gamma (NR1B3). For each subtype, there are at least two isoforms, which are generated by differential promoter usage and alternative splicing and differ only in their N-terminal regions. Retinoids, which are metabolites of vitamin A, serve as ligands for RARs. RARs function as ligand-dependent transcriptional regulators and are found to be heterodimerized with retinoid X receptors (RXRs). These transcriptionally active dimers regulate the expression of genes involved in cellular differentiation, proliferation, and apoptosis. The human retinoid X receptors are encoded by three distinct genes (RXRalpha, RXRbeta, and RXRgamma) and bind selectively and with high affinity to the vitamin A derivative, 9-cis-retinoic acid. RXRs are type-II nuclear hormone receptors that are largely localized to the nuclear compartment independent of ligand binding. Nuclear RXRs form heterodimers with nuclear hormone receptor subfamily 1 proteins, including thyroid hormone receptor, retinoic acid receptors, vitamin D receptor, peroxisome proliferator-activated receptors, liver X receptors, and farnesoid X receptor.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
nuclear receptor
NCBI Official Symbol
vdr-a
NCBI Protein Information
nuclear receptor
Protein Family
Similar Products
Product Notes
The vdr-a (Catalog #AAA2549987) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Nuclear Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the vdr-a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nuclear Receptor, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
