Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) 3T3, 3) Rat brain using LC3A Monoclonal Antibody at dilution of 1:1000.)
Autophagosome Marker Antibody Kit
Autophagosome Marker Antibody Sampler Kit
Applications
Western Blot
Synonyms
Autophagosome Marker; Autophagosome Marker Antibody Sampler Kit; Autophagosome Marker antibody kit
Applicable Applications for Autophagosome Marker antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) 3T3, 3) Rat brain using LC3A Monoclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) 3T3, 3) Rat brain using LC3A Monoclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) 3T3, 3) Rat brain with MAP LC3B Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) 3T3, 3) Rat brain with MAP LC3B Polyclonal Antibody.)
Related Product Information for Autophagosome Marker antibody kit
Background: Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10. Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule- associated proteins 1A and 1B (termed MAP1LC3) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 that is critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles. The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form LC3-II have been used as indicators of autophagy.
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Product Notes
The Autophagosome Marker (Catalog #AAA2549996) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Autophagosome Marker can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Autophagosome Marker for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Autophagosome Marker, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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