Western Blot (WB)
(Western Blot detection against Immunogen (38.06kD).)
Mouse anti-Human, Mouse ARHGEF6 Monoclonal Antibody | anti-ARHGEF6 antibody
ARHGEF6 (COOL2, KIAA0006, PIXA, Rho Guanine Nucleotide Exchange Factor 6, Alpha-Pix, COOL-2, PAK-interacting Exchange Factor alpha, Rac/Cdc42 Guanine Nucleotide Exchange Factor 6)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(Western Blot detection against Immunogen (38.06kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (38.06kD).)
Western Blot (WB)
(Western Blot analysis of ARHGEF6 expression in transfected 293T cell line by ARHGEF6 monoclonal antibody.|Lane 1: ARHGEF6 transfected lysate (Predicted MW: 87.5kD).|Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of ARHGEF6 expression in transfected 293T cell line by ARHGEF6 monoclonal antibody.|Lane 1: ARHGEF6 transfected lysate (Predicted MW: 87.5kD).|Lane 2: Non-transfected lysate.)
Immunoprecipitation (IP)
(Immunoprecipitation of ARHGEF6 transfected lysate using ARHGEF6 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ARHGEF6 monoclonal antibody.)
Immunoprecipitation (IP)
(Immunoprecipitation of ARHGEF6 transfected lysate using ARHGEF6 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ARHGEF6 monoclonal antibody.)
Testing Data
(Detection limit for recombinant GST tagged ARHGEF6 is ~0.03ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged ARHGEF6 is ~0.03ng/ml as a capture antibody.)
Testing Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC42 and ARHGEF6 HeLa cells were stained with CDC42 rabbit purified polyclonal 1:1200 and ARHGEF6 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Testing Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC42 and ARHGEF6 HeLa cells were stained with CDC42 rabbit purified polyclonal 1:1200 and ARHGEF6 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
NCBI and Uniprot Product Information
NCBI Description
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It may form a complex with G proteins and stimulate Rho-dependent signals. This protein is activated by PI3-kinase. Mutations in this gene can cause X-chromosomal non-specific mental retardation. [provided by RefSeq, Jul 2008]
Uniprot Description
ARHGEF6: Acts as a RAC1 guanine nucleotide exchange factor (GEF). Interacts with PAK kinases through the SH3 domain. Interacts with GIT1. Component of cytoplasmic complexes, which also contain PXN, GIT1 and PAK1. Ubiquitous. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; GEFs; GEFs, Rac/Rho
Chromosomal Location of Human Ortholog: Xq26.3
Cellular Component: lamellipodium; intercellular junction; intracellular; cytosol
Molecular Function: protein binding; Rho guanyl-nucleotide exchange factor activity; GTPase activator activity
Biological Process: lamellipodium biogenesis; regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; small GTPase mediated signal transduction; JNK cascade; positive regulation of GTPase activity
Disease: Mental Retardation, X-linked 46
Research Articles on ARHGEF6
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Product Notes
The ARHGEF6 arhgef6 (Catalog #AAA6001647) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ARHGEF6 (COOL2, KIAA0006, PIXA, Rho Guanine Nucleotide Exchange Factor 6, Alpha-Pix, COOL-2, PAK-interacting Exchange Factor alpha, Rac/Cdc42 Guanine Nucleotide Exchange Factor 6) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGEF6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Researchers should empirically determine the suitability of the ARHGEF6 arhgef6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QTEADCINNI NDFLKGCATL QVEIFDPDDL YSGVNFSKVL STLLAVNKAT EDQLSERPCG RSSSLSAANT SQTNPQGAVS STVSGLQRQS KTVEMTENGS HQLIV. It is sometimes possible for the material contained within the vial of "ARHGEF6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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