Arachidonate-15-Lipoxygenase Recombinant Protein | ALOX15B recombinant protein

Recombinant Arachidonate-15-Lipoxygenase, Type B (ALOX15B)

Cat. Num. AAA2031429

• Gene Names: ALOX15; 12-LOX; 15LOX-1; 15-LOX-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Arachidonate-15-Lipoxygenase; Recombinant Arachidonate-15-Lipoxygenase; Type B (ALOX15B); ALOX15B recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 6.6 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- RCHYLPKN FPVTDAMVAS VLGPGTSLQA ELEKGSLFLV DHGILSGIQT NVINGKPQFS AAPMTLLYQS PGCGPLLPLA IQLSQTPGPN SPIFLPTDDK WDWLLAKTWV RNAEFSFHEA LTHLLHSHLL PEVFTLATLR QLPHCHPLFK LLIPHTRYTL HINTLARELL IVPGQVVDRS TGIGIEGFSE LIQRNMKQLNYSLLCLPEDI RTRGVEDIPG YYYRDDGMQI WGAVERFVSE
• Sequence Length: 662

• Applicable Applications for ALOX15B recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 30.5kDa
• Accurate Molecular Mass (KD): 27kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Gln24~Ser121
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 40316937
• NCBI GeneID: 246
• NCBI Accession #: NP_001131.3
• NCBI GenBank Nucleotide #: NM_001140.3
• Molecular Weight: 70,108 Da
• NCBI Official Full Name: arachidonate 15-lipoxygenase
• NCBI Official Synonym Full Names: arachidonate 15-lipoxygenase
• NCBI Official Symbol: ALOX15
• NCBI Official Synonym Symbols: 12-LOX; 15LOX-1; 15-LOX-1
• NCBI Protein Information: arachidonate 15-lipoxygenase
• UniProt Protein Name: Arachidonate 15-lipoxygenase
• Protein Family: Arachidonate 15-lipoxygenase
• UniProt Gene Name: ALOX15
• UniProt Synonym Gene Names: LOG15; 15-LOX; 15-LOX-1; 12-LOX
• UniProt Entry Name: LOX15_HUMAN

Uniprot Description

ALOX15: Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. Belongs to the lipoxygenase family.

Protein type: Oxidoreductase; Motility/polarity/chemotaxis; Lipid Metabolism - linoleic acid; Lipid Metabolism - arachidonic acid; EC 1.13.11.31; EC 1.13.11.33

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: extrinsic to internal side of plasma membrane; membrane; plasma membrane; lipid particle; cytosol

Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; arachidonate 12-lipoxygenase activity; hepoxilin A3 synthase activity; protein binding; arachidonate 15-lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding

Biological Process: ossification; positive regulation of heterotypic cell-cell adhesion; positive regulation of actin filament polymerization; wound healing; lipoxygenase pathway; negative regulation of adaptive immune response; phosphatidylethanolamine biosynthetic process; arachidonic acid metabolic process; leukotriene metabolic process; hepoxilin biosynthetic process; inflammatory response; apoptotic cell clearance; bone mineralization