Human Arachidonate 15-Lipoxygenase ELISA Kit | 15-LO elisa kit

Human Arachidonate 15-Lipoxygenase ELISA Kit

Cat. Num. AAA761410

• Gene Names: ALOX15; 12-LOX; 15LOX-1; 15-LOX-1
• Reactivity: Human
• Synonyms: Arachidonate 15-Lipoxygenase; Human Arachidonate 15-Lipoxygenase ELISA Kit; 15-LO/ALOX15/15-LOX-1/15LOX-1/LOX15; 15-LO elisa kit

• Reactivity: Human
• Sequence Length: 662

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 62.5-4000pg/ml
• Sensitivity: 37.5pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for 15-LO elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human 15-LO antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human 15-LO antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ApoH amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human 15-LO can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 40316937
• NCBI GeneID: 246
• NCBI Accession #: NP_001131.3
• NCBI GenBank Nucleotide #: NM_001140.3
• Molecular Weight: 70,108 Da
• NCBI Official Full Name: arachidonate 15-lipoxygenase
• NCBI Official Synonym Full Names: arachidonate 15-lipoxygenase
• NCBI Official Symbol: ALOX15
• NCBI Official Synonym Symbols: 12-LOX; 15LOX-1; 15-LOX-1
• NCBI Protein Information: arachidonate 15-lipoxygenase
• UniProt Protein Name: Arachidonate 15-lipoxygenase
• Protein Family: Arachidonate 15-lipoxygenase
• UniProt Gene Name: ALOX15
• UniProt Synonym Gene Names: LOG15; 12-LOX
• UniProt Entry Name: LOX15_HUMAN

Uniprot Description

ALOX15: Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. Belongs to the lipoxygenase family.

Protein type: EC 1.13.11.33; Oxidoreductase; Lipid Metabolism - linoleic acid; Motility/polarity/chemotaxis; EC 1.13.11.31; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: cytosol; extrinsic to internal side of plasma membrane; lipid particle; membrane; plasma membrane

Molecular Function: arachidonate 12-lipoxygenase activity; arachidonate 15-lipoxygenase activity; hepoxilin A3 synthase activity; hepoxilin-epoxide hydrolase activity; iron ion binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding

Biological Process: apoptotic cell clearance; arachidonic acid metabolic process; bone mineralization; hepoxilin biosynthetic process; inflammatory response; leukotriene metabolic process; lipoxygenase pathway; negative regulation of adaptive immune response; ossification; phosphatidylethanolamine biosynthetic process; positive regulation of actin filament polymerization; positive regulation of heterotypic cell-cell adhesion; wound healing