Mouse anti-Human ANXA5 / Annexin V Monoclonal Antibody | anti-ANXA5 antibody

Mouse Monoclonal [clone 1F4-1A5] (IgG1,k) to Human ANXA5 / Annexin V

Cat. Num. AAA246686

• Gene Names: ANXA5; PP4; ANX5; ENX2; RPRGL3; HEL-S-7
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Immunoprecipitation, ELISA
• Purity: Protein A Purified
• Synonyms: ANXA5 / Annexin V; Monoclonal Antibody; Mouse Monoclonal [clone 1F4-1A5] (IgG1; k) to Human ANXA5 / Annexin V; Anti-ANXA5 / Annexin V Antibody (clone 1F4-1A5) IHC-plus; ANXA5; ANX5; Anchorin CII; Calphobindin I; CBP-I; ENX2; PP4; RPRGL3; Lipocortin V; Thromboplastin inhibitor; VAC-alpha; Vascular anticoagulant-alpha; PAP-I; Annexin A5; Annexin V; Annexin-5; Endonexin II; Human ANXA5; anti-ANXA5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1F4-1A5
• Specificity: Human Annexin V
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4.
• Concentration: 0.49 mg/ml (varies by lot)
• Sequence Length: 320

• Applicable Applications for anti-ANXA5 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), IP, WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: ANXA5 (AAH01429, 1 a.a. ~ 321 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: ANXA5 / Annexin V antibody was raised against aNXA5 (AAH01429, 1 a.a. ~ 321 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-Annexin V antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ANXA5 on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(Western blot of ANXA5 expression in transfected 293T cell line by ANXA5 monoclonal antibody (M01), clone 1F4-1A5.)

Western Blot (WB)

(ANXA5 monoclonal antibody (M01), clone 1F4-1A5 Western blot of ANXA5 expression in HeLa.)

Immunoprecipitation (IP)

(Immunoprecipitation of ANXA5 transfected lysate using anti-ANXA5 monoclonal antibody and Protein A Magnetic Bead.)

ELISA (EIA)

(Detection limit for recombinant GST tagged ANXA5 is approximately 0.03 ng/ml as a capture antibody.)

Related Product Information for anti-ANXA5 antibody

Annexin V belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin V is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation.

NCBI and Uniprot Product Information

• NCBI GI #: 4502107
• NCBI GeneID: 308
• NCBI Accession #: NP_001145.1
• NCBI GenBank Nucleotide #: NM_001154.3
• UniProt Accession #: P08758; Q6FHB3; Q6FI16; Q8WV69; Q9UDH9; D3DNW7
• Molecular Weight: 35,937 Da
• NCBI Official Full Name: annexin A5
• NCBI Official Synonym Full Names: annexin A5
• NCBI Official Symbol: ANXA5
• NCBI Official Synonym Symbols: PP4; ANX5; ENX2; RPRGL3; HEL-S-7
• NCBI Protein Information: annexin A5; CBP-I; PAP-I; VAC-alpha; annexin V; annexin-5; anchorin CII; endonexin II; lipocortin V; calphobindin I; thromboplastin inhibitor; vascular anticoagulant-alpha; epididymis secretory protein Li 7; placental anticoagulant protein 4; placental anticoagulant protein I
• UniProt Protein Name: Annexin A5
• Protein Family: Annexin
• UniProt Gene Name: ANXA5
• UniProt Synonym Gene Names: ANX5; ENX2; PP4; CBP-I; PP4; PAP-I; VAC-alpha
• UniProt Entry Name: ANXA5_HUMAN

NCBI Description

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008]

Uniprot Description

ANXA5: a calcium/phospholipid-binding protein and an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. The binding of labeled ANXA5 to phosphatidylserine is used as a marker of apoptosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Lipid-binding; Apoptosis; Inhibitor; Calcium-binding

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: focal adhesion; membrane; cytoplasm; intracellular; external side of plasma membrane

Molecular Function: phospholipase inhibitor activity; calcium-dependent phospholipid binding; phospholipid binding; calcium ion binding

Biological Process: response to organic substance; negative regulation of catalytic activity; signal transduction; blood coagulation; negative regulation of coagulation; negative regulation of apoptosis

Disease: Pregnancy Loss, Recurrent, Susceptibility To, 3