anti-Human, Mouse DNMT3L Antibody | anti-DNMT3L antibody

DNMT3L Antibody

Cat. Num. AAA8516398

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Immunogen affinity purified
• Synonyms: DNMT3L; DNMT3L Antibody; MGC1090; anti-DNMT3L antibody

• Reactivity: Human, Mouse
• Specificity: DNMT3L Antibody detects endogenous levels of total DNMT3L
• Purity/Purification: Immunogen affinity purified
• Form/Format: In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 387

• Applicable Applications for anti-DNMT3L antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (CHIP)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:20-1:50; IP: 1:20-1:50; CHIP: 1:20-1:50
• Immunogen: Recombinant protein of human DNMT3L
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of extracts of variouscell lines, using DNMT3L antibody.)

Related Product Information for anti-DNMT3L antibody

Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and development (1, 2). Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2, and DNMT3 (1, 2). DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation.DNMT3L is a catalytically inactive regulatory factor for the DNMT3A and DNMT3B de novo methyltransferases that is expressed at low levels in embryonic stem cells, testis, ovaries, and thymus (1, 2). These de novo methyltransferases consist of a heterotetrameric complex containing two molecules of DNMT3L, and either two molecules of DNMT3A or DNMT3B (3). DNMT3L contains an amino-terminal ATRX-DNMT3-DNMT3L (ADD) domain and a carboxy-terminal methyltransferase-like domain (4-7). The methyltransferase-like domain binds to DNMT3A and DNMT3B to stimulate catalytic activity by increasing the binding of S-adenosylmethionine and DNA (4, 5). The ADD domain recruits the methyltransferase complex to transcriptionally inactive regions of the genome by binding to unmethylated histone H3 Lys4 (6, 7).

NCBI and Uniprot Product Information

• NCBI GI #: 28872778
• NCBI GeneID: 29947
• NCBI Accession #: NP_037501.2
• NCBI GenBank Nucleotide #: NM_013369.3
• UniProt Accession #: Q9UJW3; Q9BUJ4; E9PB42
• Molecular Weight: 44kDa
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 3-like isoform 1
• NCBI Official Synonym Full Names: DNA methyltransferase 3 like
• NCBI Official Symbol: DNMT3L
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 3-like
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 3-like
• Protein Family: DNA (cytosine-5)-methyltransferase
• UniProt Gene Name: DNMT3L

NCBI Description

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context (). Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain (PubMed:17687327). Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases (PubMed:17687327). Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3 (PubMed:17687327). Plays a key role in embryonic stem cells and germ cells (). In germ cells, required for the methylation of imprinted loci together with DNMT3A (). In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization (). Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation (). While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes (). Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes (). Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression ().