Mouse anti-Human MME Monoclonal Antibody | anti-MME antibody

MME Antibody (Center)

Cat. Num. AAA9230971

• Gene Names: MME; NEP; SFE; CD10; CALLA; CMT2T; SCA43
• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: MME; Monoclonal Antibody; MME Antibody (Center); Neprilysin; 3.4.24.11; Atriopeptidase; Common acute lymphocytic leukemia antigen; CALLA; Enkephalinase; Neutral endopeptidase 24.11; NEP; Neutral endopeptidase; Skin fibroblast elastase; SFE; CD10; EPN; anti-MME antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 965CT9.1.1
• Specificity: This MME antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 472-505 amino acids from the Central region of human MME.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Length: 750

• Applicable Applications for anti-MME antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:4000
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Cellular Location: Cell membrane; Single-pass type II membrane protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-MME Antibody (Center) at 1:4000 dilutionLane 1: Human placenta lysateLane 2: Ramos whole cell lysateLane 3: Human kidney lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 100 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-MME antibody

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met-and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675). Involved in the degradation of atrial natriuretic factor (ANF) (PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).

Product Categories/Family for anti-MME antibody

Crown Antibodies; Primary Antibodies; Cancer; Immunology; Stem Cells

References

Letarte M.,et al.J. Exp. Med. 168:1247-1253 (1988). Shipp M.A.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:4819-4823 (1988). D'Adamio L.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:7103-7107 (1989). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 116256329
• NCBI GeneID: 4311
• NCBI Accession #: NP_000893.2
• NCBI GenBank Nucleotide #: NM_000902.3
• UniProt Accession #: P08473; Q3MIX4; A8K6U6; D3DNJ9
• Molecular Weight: 85514 Da
• NCBI Official Full Name: neprilysin
• NCBI Official Synonym Full Names: membrane metalloendopeptidase
• NCBI Official Symbol: MME
• NCBI Official Synonym Symbols: NEP; SFE; CD10; CALLA; CMT2T; SCA43
• NCBI Protein Information: neprilysin
• UniProt Protein Name: Neprilysin
• Protein Family: Neprilysin
• UniProt Gene Name: MME
• UniProt Synonym Gene Names: EPN; CALLA; NEP; Neutral endopeptidase; SFE

NCBI Description

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq, Jul 2008]

Uniprot Description

CD10: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV- inducible elastase activity toward skin preelastic and elastic fibers. Inhibited in a dose dependent manner by opiorphin. Belongs to the peptidase M13 family.

Protein type: EC 3.4.24.11; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 3q25.2

Cellular Component: axon; brush border; cytoplasm; dendrite; focal adhesion; plasma membrane; synapse; synaptic vesicle

Molecular Function: endopeptidase activity; exopeptidase activity; metalloendopeptidase activity; metallopeptidase activity; peptide binding; protein binding; zinc ion binding

Biological Process: angiotensin maturation; beta-amyloid metabolic process; creatinine metabolic process; kidney development; peptide metabolic process; proteolysis; sensory perception of pain

Disease: Charcot-marie-tooth Disease, Axonal, Type 2t; Membranous Nephropathy, Susceptibility To; Spinocerebellar Ataxia 43