A Disintegrin And Metalloprotease 10 (ADAM10) Recombinant Protein | ADAM10 recombinant protein

Recombinant A Disintegrin And Metalloprotease 10 (ADAM10)

Cat. Num. AAA2009917

• Gene Names: ADAM10; RAK; kuz; AD10; AD18; MADM; CD156c; HsT18717
• Applications: SDS-Page, Western Blot
• Purity: >90%
• Synonyms: A Disintegrin And Metalloprotease 10 (ADAM10); Recombinant A Disintegrin And Metalloprotease 10 (ADAM10); ADAM10 recombinant protein

• Host: E Coli
• Purity/Purification: >90%
• Form/Format: Freeze-dried powder
• Concentration: 150ug/mL (varies by lot)
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-R ATSGDKLNNN KFSLCSIRNI SQVLEKKRNN CFVESGQPIC GNGMVEQGEE CDCGYSDQCK DECCFDANQP EGRKCKLKPG KQCSPSQGPC CTAQCAFKSK SEKCRDDSDC AREGICNGFT ALCPASDPKP NFTDCNRHTQ VCINGQCAGS ICEKYGLEEC TCASSDGKDD KELCHVCCMK KMDPSTCAST GSVQWSRHFS GRTITLQPGS PCNDFRGYCD V
• Sequence Length: 748

• Applicable Applications for ADAM10 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Arg420~Val641 (Accession # O14672) with N-terminal His-Tag
• Subcellular Location: Cell membrane; Single-pass type I membrane protein. Endomembrane system
• Buffer: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.

Sequence

Gene Sequencing

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4557251
• NCBI GeneID: 102
• NCBI Accession #: NP_001101.1
• NCBI GenBank Nucleotide #: NM_001110.3
• UniProt Accession #: O14672; Q10742; Q92650
• Molecular Weight: Predicted Molecular Mass: 25.4kDa; Accurate Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 10
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 10
• NCBI Official Symbol: ADAM10
• NCBI Official Synonym Symbols: RAK; kuz; AD10; AD18; MADM; CD156c; HsT18717
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 10; CDw156; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; a disintegrin and metalloprotease domain 10
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 10
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM10
• UniProt Synonym Gene Names: KUZ; MADM; ADAM 10
• UniProt Entry Name: ADA10_HUMAN

NCBI Description

Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAM10: Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth- like factor, ephrin-A2 and for constitutive and regulated alpha- secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.

Protein type: Protease; Cell surface; Vesicle; Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral; EC 3.4.24.81

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: Golgi apparatus; focal adhesion; cell surface; membrane; intracellular membrane-bound organelle; cytoplasm; postsynaptic density; integral to membrane; plasma membrane; Golgi-associated vesicle; nucleus

Molecular Function: integrin binding; protein binding; protein homodimerization activity; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; endopeptidase activity; SH3 domain binding; protein kinase binding; receptor binding

Biological Process: integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; axon guidance; Notch signaling pathway; extracellular matrix organization and biogenesis; in utero embryonic development; constitutive protein ectodomain proteolysis; membrane protein ectodomain proteolysis; Notch receptor processing; negative regulation of cell adhesion; positive regulation of cell growth; protein amino acid phosphorylation; collagen catabolic process; extracellular matrix disassembly; PMA-inducible membrane protein ectodomain proteolysis; cell-cell signaling; monocyte activation; positive regulation of cell proliferation; ephrin receptor signaling pathway; positive regulation of cell migration

Disease: Reticulate Acropigmentation Of Kitamura; Alzheimer Disease 18