A Disintegrin And Metalloprotease 10 (ADAM10) Recombinant Protein | ADAM10 recombinant protein

Recombinant A Disintegrin And Metalloprotease 10 (ADAM10)

Cat. Num. AAA2009286

• Gene Names: Adam10; MADM
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: A Disintegrin And Metalloprotease 10 (ADAM10); Recombinant A Disintegrin And Metalloprotease 10 (ADAM10); ADAM10 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-TSG DKLNNNKFSL CSIRNISQVL EKKRNNCFVE SGQPICGNGM VEQGEECDCG YSDQCKDECC FDANQPEGKK CKLKPGKQCS PSQGPCCTAQ CAFKSKSEKC RDDSDCAKEG ICNGFTALCP ASDPKPNFTD CNRHTQVCIN GQCAGSICEK YDLEECTCAS SDGKDDKELC HVCCMKKMAP STCASTGSLQ WNKQFTGRTI TLQPGSPCND FRGYCDVFMR CRLVD
• Sequence Length: 544

• Applicable Applications for ADAM10 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Thr218~Asp445 (Accession # Q10743) with N-terminal His-Tag
• Subcellular Location: Membrane; Single-pass type I membrane protein
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.4
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 37096570
• NCBI GeneID: 29650
• UniProt Accession #: Q10743
• Molecular Weight: 26.5kDa
• NCBI Official Full Name: Disintegrin and metalloproteinase domain-containing protein 10
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 10
• NCBI Official Symbol: Adam10
• NCBI Official Synonym Symbols: MADM
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 10; ADAM 10; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; a disintegrin and metalloprotease domain 10; a disintegrin and metallopeptidase domain 10
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 10
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: Adam10
• UniProt Synonym Gene Names: Madm; ADAM 10
• UniProt Entry Name: ADA10_RAT

NCBI Description

may act as a disintegrin-metalloprotease related to the reprolysin family [RGD, Feb 2006]

Uniprot Description

ADAM10: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling (By similarity)

Protein type: Motility/polarity/chemotaxis; Protease; Vesicle; EC 3.4.24.81; Cell surface; Membrane protein, integral; Cell adhesion

Cellular Component: Golgi apparatus; focal adhesion; cell surface; intracellular membrane-bound organelle; membrane; postsynaptic density; cytoplasm; integral to membrane; Golgi-associated vesicle; trans-Golgi network; nucleus

Molecular Function: protein homodimerization activity; zinc ion binding; metallopeptidase activity; metal ion binding; metalloendopeptidase activity; endopeptidase activity; protein kinase binding; SH3 domain binding

Biological Process: PMA-inducible membrane protein ectodomain proteolysis; Notch signaling pathway; regulation of osteoclast differentiation; monocyte activation; in utero embryonic development; membrane protein ectodomain proteolysis; positive regulation of cell proliferation; constitutive protein ectodomain proteolysis; negative regulation of cell adhesion; proteolysis; positive regulation of cell growth; protein amino acid phosphorylation; positive regulation of cell migration