Human Adenosine Deaminase ELISA Kit | ADA elisa kit

Human Adenosine Deaminase ELISA Kit

Cat. Num. AAA8248325

• Reactivity: Human
• Synonyms: Adenosine Deaminase; Human Adenosine Deaminase ELISA Kit; ADA1; Adenosine deaminase; Adenosine aminohydrolase; ADA elisa kit

• Reactivity: Human
• Specificity: The Human Adenosine Deaminase ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Adenosine Deaminase proteins within the range of 31.2 pg/ml - 2000 pg/ml.
• Sequence Length: 363

• Samples: Cell Culture Supernates, Serum, Tissue Homogenates
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADA elisa kit

Principle of the Assay: The Human Adenosine Deaminase ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Adenosine Deaminase in Cell Culture Supernates, Serum, Tissue Homogenates. This assay employs an antibody specific for Human Adenosine Deaminase coated on a 96-well plate. Standards and samples are pipetted into the wells and Adenosine Deaminase present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Adenosine Deaminase antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Adenosine Deaminase bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.

NCBI and Uniprot Product Information

• NCBI GI #: 47078295
• NCBI GeneID: 100
• NCBI Accession #: NP_000013.2
• NCBI GenBank Nucleotide #: NM_000022.3
• UniProt Accession #: P00813; Q53F92; Q6LA59
• Molecular Weight: 40,764 Da
• NCBI Official Full Name: adenosine deaminase isoform 1
• NCBI Official Synonym Full Names: adenosine deaminase
• NCBI Official Symbol: ADA
• NCBI Protein Information: adenosine deaminase
• UniProt Protein Name: Adenosine deaminase
• Protein Family: Adenosine deaminase
• UniProt Gene Name: ADA
• UniProt Synonym Gene Names: ADA1
• UniProt Entry Name: ADA_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008]

Uniprot Description

ADA: a enzyme that converts adenosine + H2O into inosine + NH3. Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues. Genetic ADA deficiencies are a cause of autosomal recessive severe combined immuno-deficiency (SCID). Hereditary hemolytic anemia is caused by expression levels in erythrocytes 50-70 times greater than the norm.

Protein type: EC 3.5.4.4; Hydrolase; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: cell surface; cytoplasm; cytosol; external side of plasma membrane; lysosome; membrane; plasma membrane

Molecular Function: adenosine deaminase activity; protein binding; zinc ion binding

Biological Process: adenosine catabolic process; hypoxanthine salvage; inosine biosynthetic process; negative regulation of adenosine receptor signaling pathway; purine nucleotide salvage; purine salvage; regulation of cell-cell adhesion mediated by integrin; response to hypoxia; T cell activation

Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-negative, Nk Cell-negative, Due To Adenosine Deaminase Deficiency