Human adenosine deaminase ELISA Kit | ADA elisa kit

Human adenosine deaminase, ADA ELISA Kit

Cat. Num. AAA700350

• Reactivity: Human
• Synonyms: adenosine deaminase; Human adenosine deaminase; ADA ELISA Kit; adenosine aminohydrolase; ADA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human ADA. No significant cross-reactivity or interference between human ADA and analogues was observed.
• Sequence Length: 363

• Samples: Serum, urine, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 1.56 mIU/ml-100 mIU/ml
• Sensitivity: <0.39 mIU/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADA elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADA bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 47078295
• NCBI GeneID: 100
• NCBI Accession #: NP_000013.2
• NCBI GenBank Nucleotide #: NM_000022.2
• UniProt Accession #: P00813; Q53F92; Q6LA59
• Molecular Weight: 40,764 Da
• NCBI Official Full Name: adenosine deaminase
• NCBI Official Synonym Full Names: adenosine deaminase
• NCBI Official Symbol: ADA
• NCBI Protein Information: adenosine deaminase; adenosine aminohydrolase
• UniProt Protein Name: Adenosine deaminase
• Protein Family: Adenosine deaminase
• UniProt Gene Name: ADA
• UniProt Synonym Gene Names: ADA1
• UniProt Entry Name: ADA_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion.1 PublicationManual assertion based on experiment in:Ref.13

Catalytic activity: Adenosine + H2O = inosine + NH3.UniRule annotationHAMAP-Rule:MF_00540

Cofactor: Binds 1 zinc ion per subunit.1 PublicationManual assertion based on experiment in:Ref.18