Rabbit TMPRSS2 Polyclonal Antibody | anti-TMPRSS2 antibody

TMPRSS2 Antibody (Center)

Cat. Num. AAA9201009

• Gene Names: TMPRSS2; PP9284; PRSS10
• Reactivity: Human (Predicted Reactivity: Mouse)
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: TMPRSS2; Polyclonal Antibody; TMPRSS2 Antibody (Center); Transmembrane protease serine 2; 3421-; Serine protease 10; Transmembrane protease serine 2 non-catalytic chain; Transmembrane protease serine 2 catalytic chain; PRSS10; anti-TMPRSS2 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This TMPRSS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-343 amino acids from the Central region of human TMPRSS2.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 314-343
• Sequence Length: 529

• Applicable Applications for anti-TMPRSS2 antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000; FC~~1:10~50
• Function: Plasma membrane-anchored serine protease that participates in proteolytic cascades of relevance for the normal physiologic function of the prostate (PubMed: 25122198). Androgen-induced TMPRSS2 activates several substrates that include pro-hepatocyte growth factor/HGF, the protease activated receptor-2/F2RL1 or matriptase/ST14 leading to extracellular matrix disruption and metastasis of prostate cancer cells (PubMed: 15537383, PubMed: 26018085, PubMed: 25122198). In addition, activates trigeminal neurons and contribute to both spontaneous pain and mechanical allodynia (By similarity).
• Cellular Location: Cell membrane; Single-pass type II membrane protein
• Tissue Location: Expressed in several tissues that comprise large populations of epithelial cells with the highest level of transcripts measured in the prostate gland. Expressed in type II pneumocytes in the lung (at protein level). Expressed strongly in small intestine. Also expressed in colon, stomach and salivary gland.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of TMPRSS2 antibody (Center)(MBS9201009) in HepG2 cell line lysates (35ug/lane). TMPRSS2 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(TMPRSS2 Antibody (Center)(MBS9201009) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TMPRSS2 antibody

TMPRSS2 is a protein that belongs to the serine protease family. The protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. Its gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage.

Product Categories/Family for anti-TMPRSS2 antibody

Cancer; Cell biology; Neuroscience

References

Gopalan,A., Cancer Res. 69 (4), 1400-1406 (2009)
Hofer,M.D., Cancer Res. 69 (2), 640-646 (2009)

NCBI and Uniprot Product Information

• NCBI GI #: 227499990
• NCBI GeneID: 7113
• NCBI Accession #: NP_001128571.1; NP_005647.3
• NCBI GenBank Nucleotide #: NM_001135099.1
• UniProt Accession #: O15393; Q6GTK7; Q9BXX1; A8K6Z8; B2R8E5; B7Z459; D3DSJ2; F8WES1
• Molecular Weight: 53859
• NCBI Official Full Name: transmembrane protease serine 2 isoform 1
• NCBI Official Synonym Full Names: transmembrane protease, serine 2
• NCBI Official Symbol: TMPRSS2
• NCBI Official Synonym Symbols: PP9284; PRSS10
• NCBI Protein Information: transmembrane protease serine 2
• UniProt Protein Name: Transmembrane protease serine 2
• Protein Family: Transmembrane protease serine
• UniProt Gene Name: TMPRSS2
• UniProt Synonym Gene Names: PRSS10
• UniProt Entry Name: TMPS2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

TMPRSS2: a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Protein type: Protease; Membrane protein, integral; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity; scavenger receptor activity

Biological Process: protein autoprocessing; receptor-mediated endocytosis; positive regulation of virion penetration into host cell; proteolysis