Mouse Adenosine Deaminase (ADA) ELISA Kit | ADA elisa kit

Mouse Adenosine Deaminase (ADA) ELISA Kit

Cat. Num. AAA268448

• Reactivity: Mouse
• Synonyms: Adenosine Deaminase (ADA); Mouse Adenosine Deaminase (ADA) ELISA Kit; ADA elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.
• Sequence Length: 363

• Samples: Mouse serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 100 ng/mL-1.56 ng/mL
• Sensitivity: Up to 0.5 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for ADA elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse ADA monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 47078295
• NCBI GeneID: 100
• NCBI Accession #: NP_000013.2
• NCBI GenBank Nucleotide #: NM_000022.2
• Molecular Weight: 40,764 Da
• NCBI Official Full Name: adenosine deaminase
• NCBI Official Synonym Full Names: adenosine deaminase
• NCBI Official Symbol: ADA
• NCBI Protein Information: adenosine deaminase; adenosine aminohydrolase
• UniProt Protein Name: Adenosine deaminase
• Protein Family: Regulatory protein
• UniProt Gene Name: ADA
• UniProt Synonym Gene Names: ADA1
• UniProt Entry Name: ADA_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008]

Uniprot Description

ADA: a enzyme that converts adenosine + H2O into inosine + NH3. Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues. Genetic ADA deficiencies are a cause of autosomal recessive severe combined immuno-deficiency (SCID). Hereditary hemolytic anemia is caused by expression levels in erythrocytes 50-70 times greater than the norm.

Protein type: Nucleotide Metabolism - purine; EC 3.5.4.4; Hydrolase

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: dendrite cytoplasm; extracellular space; cell surface; cell soma; membrane; lysosome; cytoplasm; plasma membrane; cytosol; cell junction; external side of plasma membrane

Molecular Function: adenosine deaminase activity; protein binding; zinc ion binding; purine nucleoside binding

Biological Process: negative regulation of circadian sleep/wake cycle, non-REM sleep; T cell activation; adenosine catabolic process; deoxyadenosine catabolic process; response to morphine; positive regulation of calcium-mediated signaling; purine salvage; positive regulation of T cell differentiation in the thymus; histamine secretion; purine ribonucleoside monophosphate biosynthetic process; regulation of cell-cell adhesion mediated by integrin; response to vitamin E; positive regulation of T cell receptor signaling pathway; negative regulation of mature B cell apoptosis; positive regulation of germinal center formation; positive regulation of B cell proliferation; hypoxanthine salvage; positive regulation of smooth muscle contraction; negative regulation of adenosine receptor signaling pathway; embryonic gut development; aging; placenta development; Peyer's patch development; response to drug; dATP catabolic process; nucleobase, nucleoside and nucleotide metabolic process; positive regulation of heart rate; liver development; negative regulation of leukocyte migration; purine base metabolic process; trophectodermal cell differentiation; purine nucleotide salvage; response to hydrogen peroxide; xanthine biosynthetic process; negative regulation of inflammatory response; response to hypoxia; inosine biosynthetic process; germinal center B cell differentiation; alveolus development; positive regulation of alpha-beta T cell differentiation

Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-negative, Nk Cell-negative, Due To Adenosine Deaminase Deficiency