Bovine eukaryotic translation initiation factor 2C, 2 ELISA Kit | EIF2C2 elisa kit

Bovine Protein argonaute-2, EIF2C2 ELISA Kit

Cat. Num. AAA9319908

• Gene Names: EIF2C2; AGO2
• Reactivity: Bovine
• Synonyms: eukaryotic translation initiation factor 2C; 2; Bovine Protein argonaute-2; EIF2C2 ELISA Kit; Bovine Protein argonaute-2 (EIF2C2) ELISA kit; AGO2; MGC3183; Q10; argonaute 2; EIF2C2 elisa kit

• Reactivity: Bovine
• Specificity: No significant cross-reactivity or interference between Bovine AGO2 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-1000pg/ml.
• Sensitivity: 5.0pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated AGO2 concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine AGO2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add AGO2 and AGO2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AGO2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for EIF2C2 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated AGO2 concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine AGO2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add AGO2 and AGO2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AGO2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 45430015
• NCBI GeneID: 404130
• NCBI Accession #: NP_991363.1
• NCBI GenBank Nucleotide #: NM_205794.1
• UniProt Accession #: Q6QME8
• Molecular Weight: 97,388 Da
• NCBI Official Full Name: protein argonaute-2
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2C, 2<
• NCBI Official Symbol: EIF2C2
• NCBI Official Synonym Symbols: AGO2
• NCBI Protein Information: protein argonaute-2; eIF2C 2; eIF-2C 2; argonaute2; argonaute 2; protein slicer; argonaute RISC catalytic component 2; eukaryotic translation initiation factor 2C 2
• UniProt Protein Name: Protein argonaute-2
• UniProt Gene Name: AGO2
• UniProt Synonym Gene Names: EIF2C2; Argonaute2; eIF-2C 2; eIF2C 2
• UniProt Entry Name: AGO2_BOVIN

Uniprot Description

Function: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions UniRule annotationHAMAP-Rule:MF_03031

Catalytic activity: Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotationHAMAP-Rule:MF_03031

Cofactor: Magnesium or manganese UniRule annotationHAMAP-Rule:MF_03031