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Testing Data
Eukaryotes Tyrosinase Assay Kit
Tyrosinase Activity Assay Kit (Colorimetric)
Reactivity
Eukaryotes
Synonyms
Tyrosinase; Tyrosinase Activity Assay Kit (Colorimetric); Tyrosinase assay kit
Reactivity
Eukaryotes
Samples
Cell lysate(e.g. Melanoma cells)
Plant tissue lysate (e.g. potato)
Recombinant enzyme
Purified protein
Plant tissue lysate (e.g. potato)
Recombinant enzyme
Purified protein
Detection Method
Absorbance: 510nm
Features & Benefits
First non-radioactive assay in the market
Simple, rapid & convenient
The assay can detect Tyrosinase Activity less than 30 uU in biological samples.
Measurement of Tyrosinase Activity in various tissue/cell lysates
Analysis of Tyrosinase activity in food chemistry/skin-related research
Simple, rapid & convenient
The assay can detect Tyrosinase Activity less than 30 uU in biological samples.
Measurement of Tyrosinase Activity in various tissue/cell lysates
Analysis of Tyrosinase activity in food chemistry/skin-related research
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
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Related Product Information for Tyrosinase assay kit
Tyrosinase (EC 1.14.18.1) is a copper-binding enzyme that is expressed across a vast range of species ranging from bacteria and fungi to mammals. It is involved in two sequential reactions of the melanin synthesis pathway; first being the hydroxylation of a monophenol and second the conversion of an ortho-diphenol to a quinone. Quinone then undergoes a series of reactions including polymerization to form melanin. Tyrosinase is of great interest to the agriculture industry since it causes browning of fruits, vegetable and mushrooms, as well as to the cosmetic industry as it causes skin darkening. Development and screening of tyrosinase inhibitors, therefore is very useful for conditions such as hyperpigmentation and melasma. Tyrosinase activity is significantly increased in melanoma. Therefore, the detection of tyrosinase activity could be promising as a specific test test for melanoma and may be useful in monitoring patient response to melanoma treatments. Tyrosinase Activity Assay Kit is a simple one-step, plate-based assay for the measurement of tyrosinase activity in biological samples. In this assay, tyrosinase catalyzes the conversion of a phenolic substrate to a Quinone intermediate, which reacts with the tyrosine enhancer forming a highly stable chromophore with absorbance at 510 nm. The assay can detect as low as 30 uU Tyrosinase in biological samples
Product Categories/Family for Tyrosinase assay kit
Similar Products
Product Notes
The Eukaryotes Tyrosinase (Catalog #AAA849755) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849755 Assay Kit recognizes Eukaryotes Tyrosinase. It is sometimes possible for the material contained within the vial of "Tyrosinase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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