Rabbit Sin1 Polyclonal Antibody | anti-Sin1 antibody

Sin1 Antibody

Cat. Num. AAA841346

• Gene Names: Mapkap1; Sin1; AI591529; D230039K05Rik
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: Sin1; Polyclonal Antibody; Sin1 Antibody; MAPKAP1; MIP1; MGC2745; SIN1; SIN1b; SIN1g; anti-Sin1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti-Sin1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal; Appearance: Colorless liquid
• Concentration: 0.5mg/ml (varies by lot)
• Sequence Length: 522

• Applicable Applications for anti-Sin1 antibody: Western Blot (WB)
• Application Notes: The antibody can be used for Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Blocking peptide is available separately.
• Immunogen: Synthetic peptide surrounding amino acid 504 of mouse Sin1
• Positive Control: Jurkat (Induced and Uninduced), Rat kidney, 3T3 cell lysates
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-Sin1 antibody

Background: Stress-activated map kinase-interacting protein 1 or Sin1 is required for Rictor binding in the mTORC2 complex. Sin1 is also a scaffolding protein in SAPK (stress-activated kinase) signaling and JNK signaling pathway.

Product Categories/Family for anti-Sin1 antibody

Antibodies & Supporting Tools; Primary Antibodies (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 28893485
• NCBI GeneID: 227743
• NCBI Accession #: NP_796319
• NCBI GenBank Nucleotide #: NM_177345.4
• Molecular Weight: 37,303 Da
• NCBI Official Full Name: target of rapamycin complex 2 subunit MAPKAP1 isoform a
• NCBI Official Synonym Full Names: mitogen-activated protein kinase associated protein 1
• NCBI Official Symbol: Mapkap1
• NCBI Official Synonym Symbols: Sin1; AI591529; D230039K05Rik
• NCBI Protein Information: target of rapamycin complex 2 subunit MAPKAP1
• UniProt Protein Name: Target of rapamycin complex 2 subunit MAPKAP1
• Protein Family: Stress-activated map kinase-interacting protein
• UniProt Gene Name: Mapkap1
• UniProt Synonym Gene Names: Mip1; Sin1; TORC2 subunit MAPKAP1; SAPK-interacting protein 1
• UniProt Entry Name: SIN1_MOUSE

Uniprot Description

Sin1: a member of a conserved family of proteins that have an essential roles in signal transduction from yeast to mammals. An essential component of the mTORC2 complex, which consists of mTOR, LST8, Sin1, Protor (PRR5), and RICTOR, the Rapamycin Insensitive Companion of mTOR. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 regulates the phosphorylation of SGK1 S422 and PKCA S657. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Contains a Ras-binding (RBD) and a pleckstrin homology (PH) domains. Plays an important role in the SAPK signaling pathway by binding directly to both ATF-2 and p38. A genetic knockout of Sin1 abolished Akt-S473 phosphorylation and disrupts the RICTOR-mTOR interaction, but Akt-Thr308 phosphorylation is maintained. Six alternatively-spliced isoforms of the human protein have been reported. All isoforms except 4 can be incorporated into the the mTORC2 complex.

Protein type: Adaptor/scaffold

Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus

Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; Ras GTPase binding; phosphatidylinositol-3,4-bisphosphate binding; protein kinase binding

Biological Process: negative regulation of Ras protein signal transduction