Rabbit SREBP1 Polyclonal Antibody | anti-SREBP1 antibody

SREBP1 Antibody

Cat. Num. AAA840995

• Gene Names: Srebf1; ADD1; ADD-1; Srebp1; SREBP-1; SREBP-1c
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: SREBP1; Polyclonal Antibody; SREBP1 Antibody; Adipocyte determination- and differentiation-dependent factor 1; ADD1; Sterol regulatory element-binding transcription factor 1; anti-SREBP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti-SREBP1polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5mg/ml (varies by lot)
• Sequence Length: 1134

• Applicable Applications for anti-SREBP1 antibody: Western Blot (WB)
• Application Notes: The antibody can be used for Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Blocking peptide is available separately.
• Immunogen: Synthetic peptide surrounding amino acid 318 of rat SREBP1
• Positive Control: Jurkat (Induced and Uninduced), Rat Kidney, 3T3 cell lysates
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-SREBP1 antibody

Background: SREBP (Sterol Regulatory Element Binding Protein) 1 and 2 are transcription factors th at regulates cholesterol homeostasis. SREBP proteins are attached to the endoplasmic reticulum and nuclear envelope. SREBP proteins undergo proteolytic cleavage in response to conditions of low cellular sterol. Upon activation of SREBP 1, a soluble NH2 cleavage fragment translocate to the nucleus and activates transcription of enzymes and other proteins required for cholesterol synthesis.

Product Categories/Family for anti-SREBP1 antibody

Antibodies & Supporting Tools; Primary Antibodies (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 453040303
• NCBI GeneID: 78968
• NCBI Accession #: NP_001263636.1
• NCBI GenBank Nucleotide #: NM_001276707.1
• UniProt Accession #: P56720; Q99PI6; Q99PI7
• Molecular Weight: 118,027 Da
• NCBI Official Full Name: sterol regulatory element-binding protein 1 isoform 1
• NCBI Official Synonym Full Names: sterol regulatory element binding transcription factor 1
• NCBI Official Symbol: Srebf1
• NCBI Official Synonym Symbols: ADD1; ADD-1; Srebp1; SREBP-1; SREBP-1c
• NCBI Protein Information: sterol regulatory element-binding protein 1
• UniProt Protein Name: Sterol regulatory element-binding protein 1
• UniProt Gene Name: Srebf1
• UniProt Synonym Gene Names: Srebp1; SREBP-1; ADD1
• UniProt Entry Name: SRBP1_RAT

NCBI Description

transcription factor; binds to the sterol regulatory element 1; regulates the transcription of genes important for sterol biosynthesis [RGD, Feb 2006]

Uniprot Description

SREBP-1: Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle. Belongs to the SREBP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Membrane protein, multi-pass; Membrane protein, integral; Transcription factor

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; protein complex; intracellular membrane-bound organelle; membrane; endoplasmic reticulum; cytoplasm; integral to membrane; nucleus

Molecular Function: protein dimerization activity; sterol response element binding; DNA binding; sequence-specific DNA binding; protein complex binding; chromatin binding; transcription factor activity; protein kinase binding

Biological Process: circadian rhythm; response to peptide hormone stimulus; response to cAMP; response to glucagon stimulus; positive regulation of transcription, DNA-dependent; lipid biosynthetic process; positive regulation of cholesterol biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; response to lipid; response to organic cyclic substance; response to insulin stimulus; negative regulation of insulin secretion; positive regulation of histone deacetylation; regulation of transcription, DNA-dependent; regulation of fatty acid metabolic process; response to glucose stimulus; aging; response to drug; response to food; cholesterol metabolic process; response to retinoic acid; transcription, DNA-dependent; cellular response to starvation; cellular response to insulin stimulus; chemical signal regulation of heart rate; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; response to progesterone stimulus; regulation of insulin secretion; lung development