Target of rapamycin complex 2 subunit MAPKAP1 Recombinant Protein | MAPKAP1 recombinant protein

Recombinant Human Target of rapamycin complex 2 subunit MAPKAP1

Cat. Num. AAA1364264

• Gene Names: MAPKAP1; MIP1; SIN1; JC310; SIN1b; SIN1g
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Target of rapamycin complex 2 subunit MAPKAP1; Recombinant Human Target of rapamycin complex 2 subunit MAPKAP1; Mitogen-activated protein kinase 2-associated protein 1; Stress-activated map kinase-interacting protein 1; SAPK-interacting protein 1; mSIN1; MAPKAP1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 2-522aa; Full Length
• Sequence: AFLDNPTIILAHIRQSHVTSDDTGMCEMVLIDHDVDLEKIHPPSMPGDSGSEIQGSNGETQGYVYAQSVDITSSWDFGIRRRSNTAQRLERLRKERQNQIKCKNIQWKERNSKQSAQELKSLFEKKSLKEKPPISGKQSILSVRLEQCPLQLNNPFNEYSKFDGKGHVGTTATKKIDVYLPLHSSQDRLLPMTVVTMASARVQDLIGLICWQYTSEGREPKLNDNVSAYCLHIAEDDGEVDTDFPPLDSNEPIHKFGFSTLALVEKYSSPGLTSKESLFVRINAAHGFSLIQVDNTKVTMKEILLKAVKRRKGSQKVSGPQYRLEKQSEPNVAVDLDSTLESQSAWEFCLVRENSSRADGVFEEDSQIDIATVQDMLSSHHYKSFKVSMIHRLRFTTDVQLGISGDKVEIDPVTNQKASTKFWIKQKPISIDSDLLCACDLAEEKSPSHAIFKLTYLSNHDYKHLYFESDAATVNEIVLKVNYILESRASTARADYFAQKQRKLNRRTSFSFQKEKKSGQQ
• Sequence Length: 522

• Species: Homo sapiens (Human)
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for MAPKAP1 recombinant protein

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.

Product Categories/Family for MAPKAP1 recombinant protein

Signal Transduction

References

"Alternative polyadenylation and splicing of mRNAs transcribed from the human Sin1 gene." Schroder W., Cloonan N., Bushell G., Sculley T. Gene 339:17-23(2004)

NCBI and Uniprot Product Information

• NCBI GI #: 56788407
• NCBI GeneID: 79109
• NCBI Accession #: NP_001006618.1
• NCBI GenBank Nucleotide #: NM_001006617.1
• UniProt Accession #: Q9BPZ7; Q00426; Q5JSV5; Q5JSV6; Q5JSV9; Q658R0; Q699U1; Q699U2; Q699U3; A8K1Z5; B1AMA4; B7Z309
• Molecular Weight: 75 kDa
• NCBI Official Full Name: target of rapamycin complex 2 subunit MAPKAP1 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase associated protein 1
• NCBI Official Symbol: MAPKAP1
• NCBI Official Synonym Symbols: MIP1; SIN1; JC310; SIN1b; SIN1g
• NCBI Protein Information: target of rapamycin complex 2 subunit MAPKAP1
• UniProt Protein Name: Target of rapamycin complex 2 subunit MAPKAP1
• Protein Family: Target of rapamycin complex
• UniProt Gene Name: MAPKAP1
• UniProt Synonym Gene Names: MIP1; SIN1; TORC2 subunit MAPKAP1; SAPK-interacting protein 1; mSIN1
• UniProt Entry Name: SIN1_HUMAN

NCBI Description

This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Sin1: a member of a conserved family of proteins that have an essential roles in signal transduction from yeast to mammals. An essential component of the mTORC2 complex, which consists of mTOR, LST8, Sin1, Protor (PRR5), and RICTOR, the Rapamycin Insensitive Companion of mTOR. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 regulates the phosphorylation of SGK1 S422 and PKCA S657. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Contains a Ras-binding (RBD) and a pleckstrin homology (PH) domains. Plays an important role in the SAPK signaling pathway by binding directly to both ATF-2 and p38. A genetic knockout of Sin1 abolished Akt-S473 phosphorylation and disrupts the RICTOR-mTOR interaction, but Akt-Thr308 phosphorylation is maintained. Six alternatively-spliced isoforms of the human protein have been reported. All isoforms except 4 can be incorporated into the the mTORC2 complex.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: cytoplasm; cytoplasmic membrane-bound vesicle; cytosol; Golgi apparatus; nucleoplasm; nucleus; plasma membrane; TORC2 complex

Molecular Function: phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; protein kinase binding; Ras GTPase binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; negative regulation of Ras protein signal transduction; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; substantia nigra development; T cell costimulation; vascular endothelial growth factor receptor signaling pathway