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Western Blot (WB) (Western blot analysis of Sin1 in lysates of MCF7?, using Sin1 Antibody.)

Rabbit Sin1 Polyclonal Antibody | anti-Sin1 antibody

Sin1 Antibody

Gene Names
MAPKAP1; MIP1; SIN1; JC310; SIN1b; SIN1g
Reactivity
Human, Mouse
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
Sin1; Polyclonal Antibody; Sin1 Antibody; MAPKAP 1; MAPKAP1; MEKK2 interacting protein 1; MGC2745; MIP 1; MIP1; Mitogen activated protein kinase associated protein 1; Mitogen-activated protein kinase 2-associated protein 1; mSIN1; OTTHUMP00000064207; Ras inhibitor MGC2745; SAPK interacting protein 1; SAPK-interacting protein 1; SIN 1; SIN1_HUMAN; SIN1b; SIN1g; Stress activated map kinase interacting protein 1; Stress activated protein kinase interacting 1; Stress-activated map kinase-interacting protein 1; Target of rapamycin complex 2 subunit MAPKAP1; TORC2 subunit MAPKAP1; anti-Sin1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
Sin1 Antibody detects endogenous levels of Sin1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
522
Applicable Applications for anti-Sin1 antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA (Peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human Sin1.
Tissue Specificity
Ubiquitously expressed, with highest levels in heart and skeletal muscle.
Subcellular Location
Plasma Membrane; Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of Sin1 in lysates of MCF7?, using Sin1 Antibody.)

Western Blot (WB) (Western blot analysis of Sin1 in lysates of MCF7?, using Sin1 Antibody.)
Related Product Information for anti-Sin1 antibody
Description: Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.

Subunit Structure: All isoforms except isoform 4 can be incorporated into the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Interacts with ATF2, MAP3K2 and MAPK8. Interacts with GTP-bound HRAS and KRAS. Interacts with IFNAR2 and SGK1. Isoform 2 interacts with NBN. Isoform 1 interacts with CCDC28B.

Similarity: Belongs to the SIN1 family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 59 kDa
Predicted: 60 kDa
NCBI Official Full Name
Target of rapamycin complex 2 subunit MAPKAP1
NCBI Official Synonym Full Names
MAPK associated protein 1
NCBI Official Symbol
MAPKAP1
NCBI Official Synonym Symbols
MIP1; SIN1; JC310; SIN1b; SIN1g
NCBI Protein Information
target of rapamycin complex 2 subunit MAPKAP1
UniProt Protein Name
Target of rapamycin complex 2 subunit MAPKAP1
UniProt Gene Name
MAPKAP1
UniProt Synonym Gene Names
MIP1; SIN1; TORC2 subunit MAPKAP1; SAPK-interacting protein 1; mSIN1
UniProt Entry Name
SIN1_HUMAN

NCBI Description

This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Sin1: a member of a conserved family of proteins that have an essential roles in signal transduction from yeast to mammals. An essential component of the mTORC2 complex, which consists of mTOR, LST8, Sin1, Protor (PRR5), and RICTOR, the Rapamycin Insensitive Companion of mTOR. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 regulates the phosphorylation of SGK1 S422 and PKCA S657. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Contains a Ras-binding (RBD) and a pleckstrin homology (PH) domains. Plays an important role in the SAPK signaling pathway by binding directly to both ATF-2 and p38. A genetic knockout of Sin1 abolished Akt-S473 phosphorylation and disrupts the RICTOR-mTOR interaction, but Akt-Thr308 phosphorylation is maintained. Six alternatively-spliced isoforms of the human protein have been reported. All isoforms except 4 can be incorporated into the the mTORC2 complex.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-3,4,5-triphosphate binding; Ras GTPase binding; phosphatidylinositol-3,4-bisphosphate binding; protein kinase binding

Biological Process: epidermal growth factor receptor signaling pathway; substantia nigra development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; T cell costimulation; innate immune response; negative regulation of Ras protein signal transduction; vascular endothelial growth factor receptor signaling pathway

Research Articles on Sin1

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Product Notes

The Sin1 mapkap1 (Catalog #AAA9611224) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Sin1 Antibody reacts with Human, Mouse Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's Sin1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the Sin1 mapkap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sin1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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