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Testing Data

General Methionine Adenosyltransferase Assay Kit

Methionine Adenosyltransferase Activity Assay Kit (Colorimetric)

Reactivity
General
Synonyms
Methionine Adenosyltransferase; Methionine Adenosyltransferase Activity Assay Kit (Colorimetric); Methionine Adenosyltransferase assay kit
Ordering
For Research Use Only!
Reactivity
General
Specificity
Rapid assessment of MAT activity in biological samples or recombinant MAT preparations
Detection Method
Absorbance 570nM
Sample Type
Human or animal soft tissue (i.e. brain, liver, lung) homogenates, Cultured cell lysates (adherent or suspension cells), Heterologously expressed recombinant MAT preparations
Features & Benefits
Does not require complicated sample processing. The limit of quantification of 2 mU MAT activity per well. Simple to perform. The assay is high-throughput adaptable. The assay is homogeneous
Preparation and Storage
Store at -20 degree C
Shipping: Gel Pack
Shelf Life: Est.~12 months

Testing Data

Testing Data
Related Product Information for Methionine Adenosyltransferase assay kit
Methionine Adenosyltransferases (MATs, EC 2.5.1.6), also known as Adenosylmethionine Synthetases are a family of enzymes that synthesize S-adenosyl-L-methionine (SAMe) from L-methionine and ATP. SAMe is a primary biochemical alkylation agent (one of only two possible methyl group donors) and is also a vital metabolic precursor of the transsulfuration and polyamine synthesis (aminopropylation) pathways. All organisms express at least one MAT enzyme. In mammals, three isozymes of MAT have been identified. MAT1 and MAT3 isozymes are predominantly expressed in the liver, whereas MAT2A is expressed in most tissues. Upregulation of the MAT2A isozyme has been linked to several human diseases. MAT2A has become a popular drug target for novel cancer therapeutics as well as for hepatic fibrosis and non-alcoholic fatty liver disease. Methionine Adenosyltransferase Activity Assay Kit enables the rapid measurement of MAT activity in complex biological matrices. The assay is based on the detection of pyrophosphate, which is generated stoichiometrically during the generation of SAMe. Pyrophosphate is enzymatically metabolized to an intermediate product, which reacts with the probe to form a stable chromophore that is detected by absorbance at 570nM. The assay is homogeneous, simple to perform and does not require complicated sample processing. The assay is high-throughput adaptable and has a limit of quantification of 2 mU MAT activity per well.

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Product Notes

The General Methionine Adenosyltransferase (Catalog #AAA8400618) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8400618 Assay Kit recognizes General Methionine Adenosyltransferase. It is sometimes possible for the material contained within the vial of "Methionine Adenosyltransferase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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