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Western Blot (WB) (Anti-CEACAM1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:58 kDaObserved band size:100 kDa)

Rabbit anti-Human CEACAM1 Polyclonal Antibody | anti-CEACAM1 antibody

Anti-CEACAM1 Antibody, Rabbit Polyclonal

Gene Names
CEACAM1; BGP; BGP1; BGPI
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Protein A
Synonyms
CEACAM1; Polyclonal Antibody; Anti-CEACAM1 Antibody; Rabbit Polyclonal; CEACAM1/CD66a Antibody; Rabbit PAb; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); Anti-BGP Antibody; Anti-BGP1 Antibody; Anti-BGPI Antibody; Anti-CD66a Antibody; Anti-CEACAM-1 Antibody; anti-CEACAM1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CEACAM1/CD66a
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CEACAM1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA: 1:1000-1:2000
Immunogen
Recombinant Human CEACAM1 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human CEACAM1 extracellular domain (rhCEACAM1). Total IgG was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CEACAM1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:58 kDaObserved band size:100 kDa)

Western Blot (WB) (Anti-CEACAM1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:58 kDaObserved band size:100 kDa)
Related Product Information for anti-CEACAM1 antibody
The carcinoembryonic-antigen-related cell-adhesion molecule (CEACAM) family of proteins has been implicated in various intercellular-adhesion and intracellular-signalling-mediated effects that govern the growth and differentiation of normal and cancerous cells. CEACAM1, also known as biliary glycoprotein I (BGP I) and CD66a, is a member of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily. The highly glycosylated CEACAM1 contains one N-terminal V-type Ig-like domain and three C2-type Ig-like domains within its ECD, and one ITIM motif and a calmodulin binding site in the cytoplasmic region. CEACAM1 is a surface glycoprotein expressed on various blood cells, epithelial cells, and vascular cells. It was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.
Product Categories/Family for anti-CEACAM1 antibody
References
Gray-Owen SD, et al. (2006) CEACAM1: contact-dependent control of immunity. Nat Rev Immunol. 6(6): 433-46.Gu A, et al. (2009) Role of Ceacam1 in VEGF induced vasculogenesis of murine embryonic stem cell-derived embryoid bodies in 3D culture. Exp Cell Res. 315(10): 1668-82.Wong C, et al. (2009) CEACAM1 negatively regulates platelet-collagen interactions and thrombus growth in vitro and in vivo. Blood. 113(8): 1818-28.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
634
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,871 Da
NCBI Official Full Name
carcinoembryonic antigen-related cell adhesion molecule 1 isoform 2
NCBI Official Synonym Full Names
carcinoembryonic antigen related cell adhesion molecule 1
NCBI Official Symbol
CEACAM1
NCBI Official Synonym Symbols
BGP; BGP1; BGPI
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 1
UniProt Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 1
Protein Family
UniProt Gene Name
CEACAM1
UniProt Synonym Gene Names
BGP; BGP1; BGP-1
UniProt Entry Name
CEAM1_HUMAN

NCBI Description

This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of all variants has not been defined. [provided by RefSeq, May 2010]

Uniprot Description

CEACAM1: carcinoembryonic antigen-related cell adhesion molecule 1. A cell-cell adhesion molecule that directly associates with annexin II. Has tumor suppressor activity in prostate carcinoma. Belongs to the immunoglobulin superfamily and the CEA family. Abundantly expressed in epithelia, vessel endothelia, trophoblasts, platelets and neutrophilic granulocytes. Also present in many cell types of the immune system such as B cells, T cells, NK cells, macrophages and dendritic cells. Five alternatively spliced isoforms have been described. Two isoforms are type I membrane proteins, while three are secreted.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Immunoglobulin superfamily; Cell adhesion

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: membrane; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: protein binding; protein homodimerization activity

Biological Process: integrin-mediated signaling pathway; cell migration; angiogenesis; homophilic cell adhesion; blood coagulation; leukocyte migration

Research Articles on CEACAM1

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Product Notes

The CEACAM1 ceacam1 (Catalog #AAA8104075) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CEACAM1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CEACAM1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000 ELISA: 1:1000-1:2000. Researchers should empirically determine the suitability of the CEACAM1 ceacam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEACAM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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