Rabbit anti-Human RBM3 Polyclonal Antibody | anti-RBM3 antibody

RBM3, ID (Putative RNA-binding Protein 3, RNA-binding Motif Protein 3, RNPL) (MaxLight 405)

Cat. Num. AAA6497977

• Gene Names: RBM3; RNPL; IS1-RNPL
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: RBM3; Polyclonal Antibody; ID (Putative RNA-binding Protein 3; RNA-binding Motif Protein 3; RNPL) (MaxLight 405); anti-RBM3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RBM3.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-RBM3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human RBM3 (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RBM3 antibody (Center) in NIH-3T3 cell line lysates (35ug/lane). RBM3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(RBM3 Antibody (Center) (RB18776) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RBM3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RBM3 Antibody (Center) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of RBM3 (arrow) using rabbit polyclonal RBM3 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RBM3 gene.)

Product Categories/Family for anti-RBM3 antibody

Antibodies; Cancer Markers

References

1. Zeng, Y., J. Cell. Biochem. 107(1), 179-188 (2009). 2. Sureban, S.M., Oncogene 27(33), 4544-4556 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 5803137
• NCBI GeneID: 5935
• NCBI Accession #: NP_006734
• NCBI GenBank Nucleotide #: NM_006743.4
• UniProt Accession #: P98179
• Molecular Weight: 19 kDa (180aa) confirmed by MALDI-TOF
• NCBI Official Full Name: RNA-binding protein 3
• NCBI Official Synonym Full Names: RNA binding motif protein 3
• NCBI Official Symbol: RBM3
• NCBI Official Synonym Symbols: RNPL; IS1-RNPL
• NCBI Protein Information: RNA-binding protein 3
• UniProt Protein Name: RNA-binding protein 3
• Protein Family: RNA-binding protein
• UniProt Gene Name: RBM3
• UniProt Synonym Gene Names: RNPL
• UniProt Entry Name: RBM3_HUMAN

NCBI Description

This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2008]

Uniprot Description

RBM3: Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation.

Protein type: Ribosomal; Translation; RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: Xp11.2

Cellular Component: cytoplasm; dendrite; nucleus; large ribosomal subunit

Molecular Function: protein binding; RNA binding; nucleotide binding; ribosomal large subunit binding

Biological Process: RNA processing; regulation of translation; miRNA-mediated gene silencing, production of miRNAs; positive regulation of translation; translation; response to cold