Rabbit RICK Polyclonal Antibody | anti-RIPK2 antibody

RICK Antibody

Cat. Num. AAA151211

• Gene Names: RIPK2; CCK; RICK; RIP2; CARD3; GIG30; CARDIAK
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: RICK Antibody is affinity chromatography purified via peptide column.
• Synonyms: RICK; Polyclonal Antibody; RICK Antibody; CCK; RIP2; CARD3; GIG30; CARDIAK; UNQ277/PRO314/PRO34092; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; CARD-containing IL-1 beta ICE-kinase; receptor-interacting serine-threonine kinase 2; anti-RIPK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: RICK Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Sequence Length: 540

• Applicable Applications for anti-RIPK2 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: RICK antibody can be used for detection of RICK by Western blot at 1 mug/mL. An approximately 60 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: RICK antibody was raised against a peptide corresponding to amino acids near the amino terminus of human RICK.
• Buffer: RICK Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: RICK antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of RICK in (A) HeLa, (B) Ramos and (C) EL4 cell lysate with RICK antibody at 1 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of RICK in A431 cells with RICK antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of RICK in K562 cells with RICK antibody at 20 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of RICK in K562 cells with RICK antibody at 20 μg/mL.Red: RICK Antibody (2075)Blue: DAPI staining)

Immunocytochemistry (ICC)

(Immunocytochemistry of RICK in K562 cells with RICK antibody at 2.5 μg/mL.)

Related Product Information for anti-RIPK2 antibody

RICK Antibody: Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase was recently identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively. RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induced apoptosis and activation of NF-κB and JNK. RICK interacts with members of the TRAF family, CLARP and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of proinflammatory cytokine IL-1β. The messenger RNA of RICK is expressed in multiple human tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 20455217
• NCBI GeneID: 8767
• UniProt Accession #: O43353; Q6UWF0; B7Z748
• Molecular Weight: 60 kDa
• NCBI Official Full Name: Receptor-interacting serine/threonine-protein kinase 2
• NCBI Official Synonym Full Names: receptor-interacting serine-threonine kinase 2
• NCBI Official Symbol: RIPK2
• NCBI Official Synonym Symbols: CCK; RICK; RIP2; CARD3; GIG30; CARDIAK
• NCBI Protein Information: receptor-interacting serine/threonine-protein kinase 2; RIP-2; CARD-carrying kinase; growth-inhibiting gene 30; tyrosine-protein kinase RIPK2; receptor-interacting protein 2; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase; receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase
• UniProt Protein Name: Receptor-interacting serine/threonine-protein kinase 2
• Protein Family: Receptor-interacting serine/threonine-protein kinase
• UniProt Gene Name: RIPK2
• UniProt Synonym Gene Names: CARDIAK; RICK; RIP2; CARD-containing IL-1 beta ICE-kinase; RIP-2
• UniProt Entry Name: RIPK2_HUMAN

NCBI Description

This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. [provided by RefSeq, Jul 2008]

Uniprot Description

RIPK2: a tyrosine kinase-like kinase of the RIPK family. Activates pro-caspase-1 and pro-caspase-8. Potentiates casp-8-mediated apoptosis. May activate NF-kappaB.

Protein type: EC 2.7.10.2; Protein kinase, TKL; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; TKL group; RIPK family

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: cytoskeleton; protein complex; cytoplasm; cytosol; vesicle

Molecular Function: protein serine/threonine kinase activity; protein binding; LIM domain binding; signal transducer activity; protein homodimerization activity; CARD domain binding; non-membrane spanning protein tyrosine kinase activity; ATP binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; activation of MAPK activity; positive regulation of apoptosis; positive regulation of interleukin-12 production; apoptosis; stress-activated MAPK cascade; positive regulation of JNK cascade; toll-like receptor 3 signaling pathway; signal transduction; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; T cell proliferation; toll-like receptor 5 signaling pathway; response to exogenous dsRNA; positive regulation of immature T cell proliferation; lipopolysaccharide-mediated signaling pathway; positive regulation of interferon-beta production; JNK cascade; inflammatory response; toll-like receptor 4 signaling pathway; positive regulation of interferon-alpha production; adaptive immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of peptidyl-serine phosphorylation; positive regulation of chemokine production; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; positive regulation of interferon-gamma production; positive regulation of protein ubiquitination; positive regulation of interleukin-2 production; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of alpha-beta T cell proliferation; toll-like receptor 9 signaling pathway; positive regulation of T-helper 1 cell differentiation; positive regulation of cytokine and chemokine mediated signaling pathway; negative regulation of apoptosis