IgG2a secondary antibody
Rat IgG2a-CY3
Applications
Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Immunoprecipitation
Synonyms
IgG2a; Rat IgG2a-CY3; IgG2a secondary antibody
Host
Rat
Isotype
IgG2akappa
Clone Number
KLH/G2a-1-1
Specificity
KLH
Form/Format
Supplied in PBS/NaN3
Concentration
0.1 mg/mL (varies by lot)
Applicable Applications for IgG2a secondary antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Fluorescence-Linked Immunosorbent Assay (FLISA), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP), Blocking (BL)
Volume
1.0 mL
Conjugate
CY3 (Cyanine 3)
Preparation and Storage
Store at 2-8 degree C.
References
1. Welner R, Swett DJ, Pelsue SC. Age-related loss of bone marrow pre-B- and immature B-lymphocytes in the autoimmune-prone flaky skin mutant mice. Autoimmunity. 2005;38:399-408. (FC). 2. Gillan V, Devaney E. Regulatory T cells modulate Th2 responses induced by Brugia pahangi third-stage larvae. Infect Immun. 2005;73:4034-42. (FC). 3. Friesen C, Glatting G, Koop B, Schwarz K, Morgenstern A, Apostolidis C, et al. Breaking chemoresistance and radioresistance with [213Bi]anti-CD45 antibodies in leukemia cells. Cancer Res. 2007;67:1950-8. (FC). 4. Gillan V, Lawrence RA, Devaney E. B cells play a regulatory role in mice infected with the L3 of Brugia pahangi. Int Immunol. 2005;17:373-82. (FC). 5. Chu VT, Fröhlich A, Steinhauser G, Scheel T, Roch T, Fillatreau S, et al. Eosinophils are required for the maintenance of plasma cells in the bone marrow. Nat Immunol. 2011;12:151-60. (FC). 6. Benhamron S, Reiner I, Zcharia E, Atallah M, Grau A, Vlodavsky I, et al. Dissociation between mature phenotype and impaired transmigration in dendritic cells from heparanase-deficient mice. PLoS One. 2012;7 (5):e35602. (FC). 7. Berge T, Grønningsæter IH, Lorvik KB, Abrahamsen G, Granum S, Sundvold-Gjerstad V, et al. SH2D2A modulates T cell mediated protection to a B cell derived tumor in transgenic mice. PLoS One. 2012;7 (10):e48239. (FC). 8. Løvås T, Bruusgaard JC, Øynebråten I, Gundersen K, Bogen B. DNA vaccines: MHC II-targeted vaccine protein produced by transfected muscle fibres induces a local inflammatory cell infiltrate in mice. PLoS One. 2014;9 (10):e108069. (IHC-FS). 9. Rosenbaum M, Andreani V, Kapoor T, Herp S, Flach H, Duchniewicz M, et al. MZB1 is a GRP94 cochaperone that enables proper immunoglobulin heavy chain biosynthesis upon ER stress. Genes Dev. 2014;28:1165-78. (IP). 10. Talkington J, Nickell SP. Role of Fc gamma receptors in triggering host cell activation and cytokine release by Borrelia burgdorferi. Infect Immun. 2001;69:413-9. (Block). 11. Mayo L, Jacob-Hirsch J, Amariglio N, Rechavi G, Moutin M, Lund FE, et al. Dual role of CD38 in microglial activation and activation-induced cell death. J Immunol. 2008;181:92-103. (In vitro control). 12. Kaplan RN, Riba RD, Zacharoulis S, Bramley AH, Vincent L, Costa C, et al. VEGFR1-positive haematopoietic bone marrow progenitors initiate the pre-metastatic niche. Nature. 2005;438:820-7. (In vivo control). 13. Etich J, Bergmeier V, Frie C, Kreft S, Bengestrate L, Eming S, et al. PECAM1+/Sca1+/CD38+ vascular cells transform into myofibroblast-like cells in skin wound repair. PloS One. 2013;8 (1):e53262. (In vivo control).
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Product Notes
The IgG2a (Catalog #AAA679268) is a Secondary Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IgG2a can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Fluorescence-Linked Immunosorbent Assay (FLISA), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP), Blocking (BL). Researchers should empirically determine the suitability of the IgG2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG2a, Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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