Casein Kinase 2, active Active Protein | AT2G23070 active protein
Casein Kinase 2, active (CK2) Recombinant
Gene Names
AT2G23070; F21P24.13; F21P24_13
Purity
Highly Purified~80%, Purified by Ni2+/NTA-agarose, the b subunit using glutathioneagarose.
Synonyms
Casein Kinase 2; active; active (CK2) Recombinant; AT2G23070 active protein
Purity/Purification
Highly Purified
~80%, Purified by Ni2+/NTA-agarose, the b subunit using glutathioneagarose.
~80%, Purified by Ni2+/NTA-agarose, the b subunit using glutathioneagarose.
Form/Format
Supplied as a liquid in 20mM MOPS, pH 7.0, 1mM EDTA, 0.1mM Triton X-100, 0.3% 2-mercaptoethanol, 1mM benzamidine, 50% glycerol.
Application Notes
Suitable for use in Kinase Assays and MS Tryptic Fingerprint.
Specific Activity
~2000u/mg
Unit Definition; 1unit of human Casein Kinase 2ab, active acitivity is 1nmol phosphate incorporated into 165uM casein kinase 2 substrate peptide (RRRDDDSDDD) per minute at 30 degree C with a final ATP concentration of 100uM.
Unit Definition; 1unit of human Casein Kinase 2ab, active acitivity is 1nmol phosphate incorporated into 165uM casein kinase 2 substrate peptide (RRRDDDSDDD) per minute at 30 degree C with a final ATP concentration of 100uM.
Preparation and Storage
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for AT2G23070 active protein
Fully active CK-2; heterotetramer of 48.7kD a-subunit and 52.6kD b-subunit (a2b2).
Product Categories/Family for AT2G23070 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,236 Da
NCBI Official Full Name
casein kinase 2, alpha polypeptide
NCBI Official Symbol
AT2G23070
NCBI Official Synonym Symbols
F21P24.13; F21P24_13
NCBI Protein Information
casein kinase 2, alpha polypeptide
UniProt Protein Name
Casein kinase II subunit alpha, chloroplastic
UniProt Entry Name
CSK2P_ARATH
Uniprot Description
Function: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha chain contains the catalytic site. May act as an ectokinase that phosphorylates several extracellular proteins
By similarity.
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Subunit structure: Tetramer of two alpha and two beta chains
Probable.
Subcellular location: Plastid › chloroplast
Potential.
Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.Contains 1 protein kinase domain.
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Product Notes
The AT2G23070 (Catalog #AAA636004) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in Kinase Assays and MS Tryptic Fingerprint. Researchers should empirically determine the suitability of the AT2G23070 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Casein Kinase 2, active, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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