Mouse MME (Neprilysin) ELISA Kit | MME elisa kit

Mouse MME (Neprilysin) ELISA Kit

Cat. Num. AAA7612386

• Gene Names: Mme; NEP; SFE; CD10; CALLA; C85356; 6030454K05Rik
• Reactivity: Mouse
• Synonyms: MME (Neprilysin); Mouse MME (Neprilysin) ELISA Kit; Neprilysin; Atriopeptidase; Enkephalinase; Neutral endopeptidase 24.11; NEP; Neutral endopeptidase; Skin fibroblast elastase; SFE; CD10; Mme; MME elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of MME. No significant cross-reactivity or interference between MME and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MME elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 51338732
• NCBI GeneID: 17380
• UniProt Accession #: Q61391; Q6NXX5; Q8K251
• Molecular Weight: 85,702 Da
• NCBI Official Full Name: Neprilysin
• NCBI Official Synonym Full Names: membrane metallo endopeptidase
• NCBI Official Symbol: Mme
• NCBI Official Synonym Symbols: NEP; SFE; CD10; CALLA; C85356; 6030454K05Rik
• NCBI Protein Information: neprilysin; enkephalinase; atriopeptidase; skin fibroblast elastase; neutral endopeptidase 24.11; common acute lymphoblastic leukemia antigen
• UniProt Protein Name: Neprilysin
• Protein Family: Neprilysin
• UniProt Gene Name: Mme
• UniProt Synonym Gene Names: NEP; Neutral endopeptidase; SFE
• UniProt Entry Name: NEP_MOUSE

Uniprot Description

CD10: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV- inducible elastase activity toward skin preelastic and elastic fibers. Inhibited in a dose dependent manner by opiorphin. Belongs to the peptidase M13 family.

Protein type: Membrane protein, integral; Protease; EC 3.4.24.11

Cellular Component: synaptic vesicle; focal adhesion; membrane; axon; cytoplasm; dendrite; plasma membrane; integral to membrane; synapse; brush border

Molecular Function: peptidase activity; metallopeptidase activity; hydrolase activity; zinc ion binding; metalloendopeptidase activity; metal ion binding; endopeptidase activity; exopeptidase activity; peptide binding

Biological Process: peptide metabolic process; beta-amyloid metabolic process; creatinine metabolic process; sensory perception of pain; proteolysis