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Mouse anti-Human ALOX15 Monoclonal Antibody | anti-ALOX15 antibody

ALOX15 (Arachidonate 15-lipoxygenase, 15-Lipoxygenase 1, 15-Lipoxygenase Reticulocyte Arachidonate, 15-LOX, Arachidonate omega-6 Lipoxygenase)

Gene Names
ALOX15; 15LOX-1; 15-LOX-1
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified
Purified.
Synonyms
ALOX15; Monoclonal Antibody; ALOX15 (Arachidonate 15-lipoxygenase; 15-Lipoxygenase 1; 15-Lipoxygenase Reticulocyte Arachidonate; 15-LOX; Arachidonate omega-6 Lipoxygenase); Anti -ALOX15 (Arachidonate 15-lipoxygenase; anti-ALOX15 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
3D8
Specificity
Recognizes human ALOX15.
Purity/Purification
Purified
Purified.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Applicable Applications for anti-ALOX15 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Dilution: ELISA: 1-5ug/ml using recombinant protein. The detection limit in sandwich ELISA is ~3ng/ml.
Immunohistochemistry (Paraffin): 1.5ug/ml stained human small intestine tissue.
Western Blot: 1-5ug/ml. Band detected at ~74.67kD using HeLa NE cell lysate. Band detected at ~98.93kD using immunogen protein lysate.
Immunogen
ALOX15 (AAH29032, 1-663aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-ALOX15 antibody
ALOX15 converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. The protein also acts on C-12 of arachidonate as well as on linoleic acid.
Product Categories/Family for anti-ALOX15 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
246
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
74,804 Da
NCBI Official Full Name
arachidonate 15-lipoxygenase
NCBI Official Synonym Full Names
arachidonate 15-lipoxygenase
NCBI Official Symbol
ALOX15
NCBI Official Synonym Symbols
15LOX-1; 15-LOX-1
NCBI Protein Information
arachidonate 15-lipoxygenase; 15-LOX; 15-lipooxygenase-1; OTTHUMP00000115936; arachidonate omega-6 lipoxygenase
UniProt Protein Name
Arachidonate 15-lipoxygenase
UniProt Gene Name
ALOX15
UniProt Synonym Gene Names
LOG15
UniProt Entry Name
LOX15_HUMAN

Uniprot Description

ALOX15: Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. Belongs to the lipoxygenase family.

Protein type: Lipid Metabolism - arachidonic acid; EC 1.13.11.31; EC 1.13.11.33; Motility/polarity/chemotaxis; Lipid Metabolism - linoleic acid; Oxidoreductase

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: extrinsic to internal side of plasma membrane; membrane; plasma membrane; lipid particle; cytosol

Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; arachidonate 12-lipoxygenase activity; protein binding; hepoxilin A3 synthase activity; arachidonate 15-lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding

Biological Process: ossification; positive regulation of heterotypic cell-cell adhesion; wound healing; positive regulation of actin filament polymerization; lipoxygenase pathway; negative regulation of adaptive immune response; phosphatidylethanolamine biosynthetic process; arachidonic acid metabolic process; leukotriene metabolic process; hepoxilin biosynthetic process; inflammatory response; apoptotic cell clearance; bone mineralization

Research Articles on ALOX15

Similar Products

Product Notes

The ALOX15 alox15 (Catalog #AAA632127) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ALOX15 (Arachidonate 15-lipoxygenase, 15-Lipoxygenase 1, 15-Lipoxygenase Reticulocyte Arachidonate, 15-LOX, Arachidonate omega-6 Lipoxygenase) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALOX15 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Immunohistochemistry and Western Blot. Dilution: ELISA: 1-5ug/ml using recombinant protein. The detection limit in sandwich ELISA is ~3ng/ml. Immunohistochemistry (Paraffin): 1.5ug/ml stained human small intestine tissue. Western Blot: 1-5ug/ml. Band detected at ~74.67kD using HeLa NE cell lysate. Band detected at ~98.93kD using immunogen protein lysate. Researchers should empirically determine the suitability of the ALOX15 alox15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALOX15, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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