Rabbit GRB10 Polyclonal Antibody | anti-GRB10 antibody

GRB10 Antibody

Cat. Num. AAA9611158

• Gene Names: GRB10; RSS; IRBP; MEG1; GRB-IR; Grb-10
• Reactivity: Human; Predicted: Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: GRB10; Polyclonal Antibody; GRB10 Antibody; grb 10; GRB IR; grb-10; GRB10 adapter protein; GRB10 adaptor protein; GRB10_HUMAN; GRBIR; Growth factor receptor bound protein 10; Growth factor receptor-bound protein 10; Insulin receptor binding protein; Insulin receptor binding protein GRB IR; Insulin receptor-binding protein Grb-IR; IRBP; KIAA0207; Maternally expressed gene 1; MEG1; RSS; anti-GRB10 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GRB10 Antibody detects endogenous levels of GRB10.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 548

• Applicable Applications for anti-GRB10 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA (Peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human GRB10.
• Tissue Specificity: Widely expressed in fetal and adult tissues, including fetal and postnatal liver, lung, kidney, skeletal muscle, heart, spleen, skin and brain.
• Subcellular Location: Cytoplasm. When complexed with NEDD4 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of GRB10 in lysates of Jurkat , using GRB10 Antibody.)

Related Product Information for anti-GRB10 antibody

Description: Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.

Post Translational Modifications: Phosphorylated on serine residues upon EGF, FGF and PDGF stimulation (By similarity). Phosphorylated at Tyr-67 by TEC.

Subunit Structure: Interacts with ligand-activated tyrosine kinase receptors, including FGFR1, INSR, IGF1R, MET and PDGFRB in a phosphotyrosine-dependent manner through the SH2 domain (By similarity). Poorly binds to the EGFR (By similarity). Directly interacts with MAP3K14/NIK and is recruited to the EGFR-ERBB2 complex. Interacts with GIGYF1/PERQ1 and GIGYF2/TNRC15 (By similarity). When unphosphorylated, interacts with AKT1 and when phosphorylated with YWHAE/14-3-3 epsilon. Interacts with NEDD4. Interacts with LRP6, thus interfering with the binding of AXIN1 to LRP6 (By similarity). Binds to activated NRAS.

Similarity: The PH domain binds relatively non-specifically to several phosphoinositides, including PI5P, PI(4,5)P2, PI(3,4)P2 and PI(3,4,5)P3, with modest affinities. Belongs to the GRB7/10/14 family.

NCBI and Uniprot Product Information

• NCBI GI #: 48762693
• NCBI GeneID: 2887
• NCBI Accession #: NP_001001549.1
• NCBI GenBank Nucleotide #: NP_001001549.1
• UniProt Accession #: Q13322
• Molecular Weight: Observed: 67 kDa; Predicted: 68 kDa
• NCBI Official Full Name: growth factor receptor-bound protein 10 isoform b
• NCBI Official Synonym Full Names: growth factor receptor bound protein 10
• NCBI Official Symbol: GRB10
• NCBI Official Synonym Symbols: RSS; IRBP; MEG1; GRB-IR; Grb-10
• NCBI Protein Information: growth factor receptor-bound protein 10
• UniProt Protein Name: Growth factor receptor-bound protein 10
• Protein Family: Growth factor receptor-bound protein
• UniProt Gene Name: GRB10
• UniProt Synonym Gene Names: GRBIR; KIAA0207
• UniProt Entry Name: GRB10_HUMAN

NCBI Description

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2010]

Uniprot Description

GRB10: an adaptor protein that inhibits insulin-stimulated IRS-PI (3)K-Akt signaling pathway by disrupting the association of IRS with the insulin receptor. Also binds activated PDGFR and EGFR. Preferentially localizes to the mitochondria, modulating the anti-apoptotic activity of mitochondrial Raf-1. Can translocate to the plasma membrane and lamellipodia. Three alternatively spliced isoforms have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7p12.2

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; SH3/SH2 adaptor activity; insulin receptor binding

Biological Process: negative regulation of glucose import; negative regulation of Wnt receptor signaling pathway; insulin-like growth factor receptor signaling pathway; insulin receptor signaling pathway; negative regulation of phosphorylation; negative regulation of insulin receptor signaling pathway; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of phosphorylation; response to insulin stimulus; negative regulation of glycogen biosynthetic process