Mouse anti-Human, Rat Serotonin Receptor 1A Monoclonal Antibody | anti-HTR1A antibody

Serotonin Receptor 1A

Cat. Num. AAA631575

• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Highly Purified; Purified by ion exchange chromatography.
• Synonyms: Serotonin Receptor 1A; Monoclonal Antibody; Anti -Serotonin Receptor 1A; anti-HTR1A antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgM,k
• Clone Number: 11C254
• Specificity: Recognizes rat Seratonin receptor 1A. Species Crossreactivity: human.
• Purity/Purification: Highly Purified; Purified by ion exchange chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.05% sodium azide.

• Applicable Applications for anti-HTR1A antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 0.5ug/ml detects Serotonin Receptor 1A on 10ug of rat brain tissue lysate. Band is observed at ~40kD. Calculated MW is 46kD.; Immunohistochemistry: 1:400 detects Serotonin Receptor 1A in normal human brain tissue.
• Immunogen: Linear peptide corresponding to rat Serotonin Receptor 1A, conjugated to KLH (NP_036717).
• Positive Control: Rat brain tissue lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot: Rat brain tissue lysate was probed with MBS631575 at 0.5ug/ml. Proteins were visualized using a goat anti-mouse IgM secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Serotonin Receptor 1A (~40kD).)

Immunohistochemistry (IHC)

(Immunohistochemistry Analysis: Paraffin-embedded normal human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:400 dilution of MBS631575 Reactivity was detected using the a detection kit. Staining pattern appears as cytoplasmic.)

Related Product Information for anti-HTR1A antibody

Serotonin Receptor 1A, also known as 5-hydroxytryptamine receptor 1A, is a member of the G protein-coupled receptor family. Serotonin Receptor 1A has been found to be expressed highly in the septum and hippocampus. Serotonin Receptor 1A seems to have an inhibitory effect on Ca2+ channels, via N-channels and L-channels. The receptor actuates voltage-sensitive slowing using the N-channels and actuates steady state inhibition using the L-channels.

Product Categories/Family for anti-HTR1A antibody

Antibodies; Abs to Serotonin Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 27461808
• NCBI GeneID: 487230
• UniProt Accession #: Q6XXX9
• Molecular Weight: 46,356 Da
• NCBI Official Full Name: serotonin receptor 1A
• NCBI Official Symbol: HTR1A
• NCBI Protein Information: 5-hydroxytryptamine receptor 1A; 5-HT-1A; 5-HT1A receptor; serotonin receptor 1A
• UniProt Protein Name: 5-hydroxytryptamine receptor 1A
• Protein Family: 5-hydroxytryptamine receptor
• UniProt Gene Name: HTR1A
• UniProt Entry Name: 5HT1A_CANFA

Uniprot Description

Function: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity

Subcellular location: Cell membrane; Multi-pass membrane protein.

Sequence similarities: Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.