Western Blot (WB)
(Anti-5HT1A Receptor antibody, MBS175587, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Human U87 Cell Lysate )
Rabbit 5HT1A Receptor Polyclonal Antibody | anti-HTR1A antibody
Anti-5HT1A Receptor antibody
Predicted to work with: Mouse
Predicted to work with: Mouse
Concentration: 0.1-0.5ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Rat
Predicted Species: Hu, Ms
Antigen Retrieval: By Heat
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Western Blot (WB)
(Anti-5HT1A Receptor antibody, MBS175587, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Human U87 Cell Lysate )
Western Blot (WB)
(Anti-5HT1A Receptor antibody, MBS175587, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Human U87 Cell Lysate )
Immunohistochemistry (IHC)
(Anti-5HT1A Receptor antibody, MBS175587, IHC(P)IHC(P): Rat Brain Tissue )
Immunohistochemistry (IHC)
(Anti-5HT1A Receptor antibody, MBS175587, IHC(P)IHC(P): Rat Brain Tissue )
Background: HTR1A(5-HYDROXYTRYPTAMINE RECEPTOR 1A), also called SEROTONIN 5-HT-1A RECEPTOR or BETA-2-ADRENERGIC RECEPTOR-LIKE PROTEIN G-21, is a subtype of 5-HT receptor that binds the endogenous neurotransmitter serotonin. It is a G protein-coupled receptor(GPCR) that is coupled to Gi/Go and mediates inhibitory neurotransmission. HTR1A denotes the human gene encoding for the receptor.The HTR1A gene is located at 5q12.3. The decreases in 5-HT-1A receptor densities correlated with decreased glucose utilization as measured by PET scan. Activation of 5-HT-1A receptors has been demonstrated to impair cognition, learning, and memory by inhibiting the release of glutamate and acetylcholine in various areas of the brain. 5-HT-1A receptors in the dorsal raphe nucleus are co-localized with neurokinin 1(NK1) receptors and have been shown to inhibit the release of substance P, their endogenous ligand.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a G protein-coupled receptor for 5-hydroxytryptamine (serotonin), and belongs to the 5-hydroxytryptamine receptor subfamily. Serotonin has been implicated in a number of physiologic processes and pathologic conditions. Inactivation of this gene in mice results in behavior consistent with an increased anxiety and stress response. Mutation in the promoter of this gene has been associated with menstrual cycle-dependent periodic fevers. [provided by RefSeq, Jun 2012]
Uniprot Description
5-HT(1A): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family. 5- hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1
Chromosomal Location of Human Ortholog: 5q11.2-q13
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: protein binding; serotonin receptor activity
Biological Process: vasoconstriction; G-protein coupled receptor protein signaling pathway; cell proliferation; behavioral fear response; serotonin receptor, adenylate cyclase inhibiting pathway; regulation of dopamine metabolic process; regulation of serotonin secretion; positive regulation of cell proliferation; regulation of hormone secretion; serotonin receptor signaling pathway; serotonin metabolic process; regulation of behavior
Disease: Periodic Fever, Menstrual Cycle-dependent
Research Articles on HTR1A
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Product Notes
The HTR1A htr1a (Catalog #AAA175587) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-5HT1A Receptor antibody reacts with Reacts with: Human, Rat Predicted to work with: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's 5HT1A Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot: Concentration: 0.1-0.5ug/ml Tested Species: Hu, Rat Predicted Species: Ms Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1ug/ml Tested Species: Rat Predicted Species: Hu, Ms Antigen Retrieval: By Heat Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the HTR1A htr1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5HT1A Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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