Mouse anti-Human, Rat PSD95, Postsynaptic Density Protein 95 Monoclonal Antibody | anti-DLG4 antibody

PSD95, Postsynaptic Density Protein 95 (PSD-95, PSD95, Post-synaptic Density Protein 95, Synapse-associated Protein 90, SAP90, SAP-90)

Cat. Num. AAA632936

• Gene Names: DLG4; PSD95; SAP90; SAP-90; FLJ97752; FLJ98574
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: PSD95; Postsynaptic Density Protein 95; Monoclonal Antibody; Postsynaptic Density Protein 95 (PSD-95; Post-synaptic Density Protein 95; Synapse-associated Protein 90; SAP90; SAP-90); Anti -PSD95; anti-DLG4 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 11C253
• Specificity: Recognizes rat PSD95. Species sequence homology: mouse and human.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150 mM sodium chloride, 0.05% sodium azide.

• Applicable Applications for anti-DLG4 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry.; Dilution: Western Blot: 0.5ug/ml detects PSD95 on 10ug of rat brain tissue lysate. Rat brain lysate was probed with N5380-25. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.; Immunohistochemistry: 1:500 detects PSD95 in rat cortex tissue.
• Immunogen: Recombinant protein corresponding to human PSD95.
• Positive Control: Rat brain tissue lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Representative lot data. |Paraffin-embedded rat cortex tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of MBS632936. Staining is seen in the neuronal cellular processes and the cytoplasm.)

Testing Data

(Representative lot data. |Rat brain tissue lysate was probed with MBS632936 (0.5ug/ml). |Proteins were visualized using an IgG (HRP) Goat Anti-Mouse and a chemiluminescence detection system. |Arrow indicates PSD95 (~95kD).)

Related Product Information for anti-DLG4 antibody

PSD95 (postsynaptic density 95) represent a class of anchoring proteins which serve to localize various neuronal ion channels to post-synaptic densities. PSD95 and related proteins contain PDZ domains, which interact with NMDA receptors and voltage-gated K+ channels via a ET/SXV motif in the cytoplasmic carboxyl terminus of the receptor channels.

Product Categories/Family for anti-DLG4 antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 3318653
• NCBI GeneID: 1742
• UniProt Accession #: O14909; P78352; Q3ZCU5
• Molecular Weight: 31,914 Da
• NCBI Official Full Name: post-synaptic density protein 95
• NCBI Official Synonym Full Names: discs, large homolog 4 (Drosophila)
• NCBI Official Symbol: DLG4
• NCBI Official Synonym Symbols: PSD95; SAP90; SAP-90; FLJ97752; FLJ98574
• NCBI Protein Information: disks large homolog 4; OTTHUMP00000166066; OTTHUMP00000166067; OTTHUMP00000166068; discs large homolog 4; Tax interaction protein 15; synapse-associated protein 90; postsynaptic density protein 95; post-synaptic density protein 95
• UniProt Protein Name: Discs, large homolog 4 (Drosophila), isoform CRA_d
• Protein Family: Disks large
• UniProt Gene Name: DLG4
• UniProt Entry Name: O14909_HUMAN

NCBI Description

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]