Western Blot (WB)
(Western Blot analysis of lysates using 1ug/ml MBS620630 Lane 1, 3: Hela cell lysate Lane 2, 4: Mouse brain tissue lysate Lane 1, 2: MBS620630 Lane 3, 4: MBS620630 pre-incubated with immunizing peptide The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.)
Rabbit anti-Human, Mouse Serotonin Receptor 3A Polyclonal Antibody
Serotonin Receptor 3A (5-HT3A, HTR3A, 5-Hydroxytryptamine (Serotonin) Receptor 3A, 5-HT-3, 5-HT3R, HTR3, 5HT3 Serotonin Receptor, Serotonin-gated Ion Channel Receptor)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Serotonin Receptor 3A; Polyclonal Antibody; Serotonin Receptor 3A (5-HT3A; HTR3A; 5-Hydroxytryptamine (Serotonin) Receptor 3A; 5-HT-3; 5-HT3R; HTR3; 5HT3 Serotonin Receptor; Serotonin-gated Ion Channel Receptor); Anti -Serotonin Receptor 3A (5-HT3A; anti-Serotonin Receptor 3A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous levels of human 5HT3A protein. Species crossreactivity: mouse
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 80ul sterile ddH2O.
Applicable Applications for anti-Serotonin Receptor 3A antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in ELISA, Western Blot and Flow Cytometry.
Dilution: ELISA: 0.05-0.2ug/ml
Western Blot: 0.5-1ug/ml
Flow Cytometry: 2-5ug for 1x10e6 cells
Dilution: ELISA: 0.05-0.2ug/ml
Western Blot: 0.5-1ug/ml
Flow Cytometry: 2-5ug for 1x10e6 cells
Immunogen
Synthetic peptide corresponding to the first extracellular domain of human 5HT3A (KLH) (Swiss Prot: P46098).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot analysis of lysates using 1ug/ml MBS620630 Lane 1, 3: Hela cell lysate Lane 2, 4: Mouse brain tissue lysate Lane 1, 2: MBS620630 Lane 3, 4: MBS620630 pre-incubated with immunizing peptide The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.)
Western Blot (WB)
(Western Blot analysis of lysates using 1ug/ml MBS620630 Lane 1, 3: Hela cell lysate Lane 2, 4: Mouse brain tissue lysate Lane 1, 2: MBS620630 Lane 3, 4: MBS620630 pre-incubated with immunizing peptide The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HepG2 cells using MBS620630 (shaded histogram) or with an isotype control antibody (I1904-30H) open histogram), followed by R-PE conjugated anti-rabbit IgG.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HepG2 cells using MBS620630 (shaded histogram) or with an isotype control antibody (I1904-30H) open histogram), followed by R-PE conjugated anti-rabbit IgG.)
Related Product Information for anti-Serotonin Receptor 3A antibody
HT receptors interact with the neurotransmitter and peripheral signal mediator serotonin. In particular, 5-hydroxytryptamine (5HT) receptors mediate the role of serotonin as an endogenous ligand and in pharmaceutical and hallucinogenic drugs. In addition, 5HT receptors affect the release and activities of other neurotransmitters, such as glutamate, dopamine, and GABA. 5HT3A receptor belongs to the serotonin receptor family and is one member of G protein coupled receptor (GPCR) family. The receptor is a ligand-gated ion channel that mediates a fast excitatory response. This receptor is also a member of the ligand-gated ion channel superfamily and is related to the nicotinic, GABAA, and NMDA receptors.
Product Categories/Family for anti-Serotonin Receptor 3A antibody
Similar Products
Product Notes
The Serotonin Receptor 3A (Catalog #AAA620630) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Serotonin Receptor 3A (5-HT3A, HTR3A, 5-Hydroxytryptamine (Serotonin) Receptor 3A, 5-HT-3, 5-HT3R, HTR3, 5HT3 Serotonin Receptor, Serotonin-gated Ion Channel Receptor) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Serotonin Receptor 3A can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in ELISA, Western Blot and Flow Cytometry. Dilution: ELISA: 0.05-0.2ug/ml Western Blot: 0.5-1ug/ml Flow Cytometry: 2-5ug for 1x10e6 cells. Researchers should empirically determine the suitability of the Serotonin Receptor 3A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Serotonin Receptor 3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
