Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded normal rat hippocampus tissue (Fig. 1 & 2) was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. In Fig. 1, MBS626374was incubated with phospho peptide and in Fig. 2, MBS626374 was incubated with the control peptide. Immunostaining was performed using a 1:25 dilution of MBS626374 Reactivity was detected using the IHC-Select® Detection Kit. The staining pattern appears as cytoplasmic (Fig. 2). The Pyramidal layer of the hippocampus stains as expected. This lot is diffusive at higher titers, specifically in the cerebellum and fimbria of the hippocampus.)
Rabbit anti-Rat NR1 Polyclonal Antibody
NR1, phosphorylated (Ser896) (NMDA Receptor, N-methyl-D-aspartate Receptor)
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
NR1; Polyclonal Antibody; phosphorylated (Ser896) (NMDA Receptor; N-methyl-D-aspartate Receptor); Anti -NR1; anti-NR1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat NR1 when phosphorylated at Ser896. Species sequence homology: human, mouse 100%.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150 mM sodium chloride, 0.05% sodium azide.
Applicable Applications for anti-NR1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1ug/ml detects NR1 on 10ug of lambda phosphatase untreated and treated rat microsomal brain tissue lysates.
Immunohistochemistry: 1:25 detects NR1 in normal rat hippocampus tissue.
Dilution: Western Blot: 1ug/ml detects NR1 on 10ug of lambda phosphatase untreated and treated rat microsomal brain tissue lysates.
Immunohistochemistry: 1:25 detects NR1 in normal rat hippocampus tissue.
Immunogen
Synthetic peptide corresponding to rat NR1 phosphorylated at Ser896 (KLH). Epitope: Phosphorylated Ser896
Positive Control
Lambda phosphatase untreated and treated rat microsomal brain tissue lysates
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded normal rat hippocampus tissue (Fig. 1 & 2) was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. In Fig. 1, MBS626374was incubated with phospho peptide and in Fig. 2, MBS626374 was incubated with the control peptide. Immunostaining was performed using a 1:25 dilution of MBS626374 Reactivity was detected using the IHC-Select® Detection Kit. The staining pattern appears as cytoplasmic (Fig. 2). The Pyramidal layer of the hippocampus stains as expected. This lot is diffusive at higher titers, specifically in the cerebellum and fimbria of the hippocampus.)
Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded normal rat hippocampus tissue (Fig. 1 & 2) was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. In Fig. 1, MBS626374was incubated with phospho peptide and in Fig. 2, MBS626374 was incubated with the control peptide. Immunostaining was performed using a 1:25 dilution of MBS626374 Reactivity was detected using the IHC-Select® Detection Kit. The staining pattern appears as cytoplasmic (Fig. 2). The Pyramidal layer of the hippocampus stains as expected. This lot is diffusive at higher titers, specifically in the cerebellum and fimbria of the hippocampus.)
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. Lambda phosphatase untreated and treated rat microsomal brain tissue lysates were probed with MBS626374 (1µg/mL); untreated (lane 1) and treated (lane 2). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates NR1 (~130 kDa).)
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. Lambda phosphatase untreated and treated rat microsomal brain tissue lysates were probed with MBS626374 (1µg/mL); untreated (lane 1) and treated (lane 2). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates NR1 (~130 kDa).)
Related Product Information for anti-NR1 antibody
NR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.
Product Categories/Family for anti-NR1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The NR1 (Catalog #AAA626374) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NR1, phosphorylated (Ser896) (NMDA Receptor, N-methyl-D-aspartate Receptor) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 1ug/ml detects NR1 on 10ug of lambda phosphatase untreated and treated rat microsomal brain tissue lysates. Immunohistochemistry: 1:25 detects NR1 in normal rat hippocampus tissue. Researchers should empirically determine the suitability of the NR1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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