Western Blot (WB)
(Immunoprecipitation of cPLA2 from untreated and TNF-alpha treated HeLa cells followed by Western blot analysis, using MBS618221.)
Rabbit c-Phospholipase A2 Polyclonal Antibody
c-Phospholipase A2 (Cytosolic PLA2, cPLA2)
Reactivity
Human, Monkey, Mouse, Rat
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by Protein A and peptide affinity chromatography.
Synonyms
c-Phospholipase A2; Polyclonal Antibody; c-Phospholipase A2 (Cytosolic PLA2; cPLA2); Anti -c-Phospholipase A2 (Cytosolic PLA2; anti-c-Phospholipase A2 antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous levels of total human cPLA2. Does not crossreact with the secreted PLA2 (sPLA2) or cytosolic Ca2+ independent PLA2 (iPLA2). Species crossreactivity: monkey, mouse, and rat.
Purity/Purification
Affinity Purified
Purified by Protein A and peptide affinity chromatography.
Purified by Protein A and peptide affinity chromatography.
Form/Format
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Applicable Applications for anti-c-Phospholipase A2 antibody
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:1000
Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking overnight .
Immunoprecipitation (IP): 1:50
Optimal dilutions to be determined by the end researcher.
Other applications have not been tested.
Dilution: Western Blot: 1:1000
Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking overnight .
Immunoprecipitation (IP): 1:50
Optimal dilutions to be determined by the end researcher.
Other applications have not been tested.
Immunogen
Synthetic peptide corresponding to the C-terminal residues of human cPLA2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Immunoprecipitation of cPLA2 from untreated and TNF-alpha treated HeLa cells followed by Western blot analysis, using MBS618221.)
Western Blot (WB)
(Immunoprecipitation of cPLA2 from untreated and TNF-alpha treated HeLa cells followed by Western blot analysis, using MBS618221.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa, C6 and NIH/3T3 cells, using MBS618221.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa, C6 and NIH/3T3 cells, using MBS618221.)
Related Product Information for anti-c-Phospholipase A2 antibody
Cytosolic phospholipase A2 (cPLA2) is a ubiquitously distributed enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and the release of arachidonic acid. cPLA2 has been implicated in diverse cellular responses such as mitogenesis, differentiation, inflammation and cytotoxicity. Calcium binding to the Nterminal CalB domain of cPLA2 promotes the translocation of cPLA2 from cytosol to membrane, where cPLA2 cleaves arachidonic acid from natural membrane. Phosphorylation of cPLA2 by MAPK (p42/44 and p38) at serine 505 and serine 727 stimulates its catalytic activity.
Product Categories/Family for anti-c-Phospholipase A2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Chain C, Phospholipase A2 (Pla2) From Naja Naja Venom
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Product Notes
The c-Phospholipase A2 (Catalog #AAA618221) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The c-Phospholipase A2 (Cytosolic PLA2, cPLA2) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's c-Phospholipase A2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). Suitable for use in ELISA, Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking overnight. Immunoprecipitation (IP): 1:50 Optimal dilutions to be determined by the end researcher. Other applications have not been tested. Researchers should empirically determine the suitability of the c-Phospholipase A2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Phospholipase A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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