Rabbit DAP1 Polyclonal Antibody | anti-DAP1 antibody

DAP1 (Death Associated Protein 1, MGC99796)

Cat. Num. AAA618157

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: DAP1; Polyclonal Antibody; DAP1 (Death Associated Protein 1; MGC99796); Anti -DAP1 (Death Associated Protein 1; anti-DAP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total DAP1 protein. It does not crossreact with other DAP family members. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-DAP1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.; Immunoprecipitation: 1:50
• Immunogen: Synthetic peptide (KLH coupled) corresponding to residues around amino acid 94 of human DAP1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of extracts from HeLa, NIH/3T3, L929, C6 and PC12 cells, using DAP1 Antibody.)

Related Product Information for anti-DAP1 antibody

Death associated protein 1 (DAP1) is a 15kD protein that functions as a positive mediator of cell death initiated by interferon-gamma (1,2). The DAP1 protein is proline rich and possesses one SH3 binding motif, as well as several consensus protein kinase phosphorylation sites (1). The protein is localized in the cytoplasm, but the detailed mechanism of its proapoptotic function is unclear.

Product Categories/Family for anti-DAP1 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 6325087
• NCBI GeneID: 855933
• NCBI Accession #: NP_015155.1
• NCBI GenBank Nucleotide #: NM_001183984.1
• UniProt Accession #: Q12091; D6W3J8
• Molecular Weight: 16,758 Da
• NCBI Official Full Name: Dap1p
• NCBI Official Symbol: DAP1
• NCBI Protein Information: Dap1p
• UniProt Protein Name: Damage response protein 1
• Protein Family: Damage response protein
• UniProt Gene Name: DAP1
• UniProt Entry Name: DAP1_YEAST

Uniprot Description

Domain: The cytochrome b5 heme-binding domain lacks the conserved iron-binding His residues at positions 81 and 105

By similarity.

Miscellaneous: Present with 6300 molecules/cell in log phase SD medium.

Sequence similarities: Belongs to the cytochrome b5 family. MAPR subfamily.Contains 1 cytochrome b5 heme-binding domain.