Mouse Bad Monoclonal Antibody

Bad, phosphorylated (Ser112) (Bcl2 Antagonist of Cell Death)

Cat. Num. AAA633399

• Reactivity: Human, Monkey, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Ascites; Ascites
• Synonyms: Bad; Monoclonal Antibody; phosphorylated (Ser112) (Bcl2 Antagonist of Cell Death); Anti -Bad; anti-Bad antibody

• Host: Mouse
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3A37
• Specificity: Detects endogenous levels of total Bad only when phosphorylated at Ser112. Does not detect Bad phosphorylated at other sites. Nor does it detect related family members. Species Crossreactivity: Human, mouse, monkey and rat. Other species have not been tested.
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.

• Applicable Applications for anti-Bad antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunoprecipitation; Dilution: Western Blot: 1:2000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.; Immunoprecipitation: 1:50; Optimal dilutions to be determined by the researcher.
• Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser112 of mouse Bad.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from COS cells untreated or TPA-treated, using MBS633399 (7E11) Monoclonal Antibody (upper) or B0003-47 lower).)

Western Blot (WB)

(Western blot analysis of extracts from 293 cells transfected with Wild-type Bad, Bad (Ser112A), Bad (S136A), Bad (S112A/S136A) and treated with TPA or forskolin, using Phospho-Bad (Ser112)(7E11) Monoclonal Antibody (upper) or Bad Antibody #9292 (lower).)

Related Product Information for anti-Bad antibody

Bad is a proapoptotic member of the Bcl-2 family that can displace Bax from binding to Bcl-2 and Bcl-XL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136. Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-2 and Bcl-XL. Akt has been shown to promote cell survival via its ability to phosphorylate Bad at Ser136. Ser112 has been shown to be the substrate in vivo and in vitro of p90RSK and mitochondria-anchored PKA. Phosphorylation of Ser155 by PKA plays a critical role in blocking the dimerization of Bad to Bcl-XL because of its position in the BH3 domain.

Product Categories/Family for anti-Bad antibody

Antibodies; Abs to Apoptosis